Journal Article

Calculating the most likely intron splicing orders in S. pombe, fruit fly, Arabidopsis thaliana, and humans

Li, Meng

2020

Overview

Background Introns have been shown to be spliced in a defined order, and this order influences both alternative splicing regulation and splicing fidelity, but previous studies have only considered neighbouring introns. The detailed intron splicing order remains unknown. Results In this work, a method was developed that can calculate the intron splicing orders of all introns in each transcript. A simulation study showed that this method can accurately calculate intron splicing orders. I further applied this method to real S. pombe , fruit fly, Arabidopsis thaliana , and human sequencing datasets and found that intron splicing orders change from gene to gene and that humans contain more not in-order spliced transcripts than S. pombe , fruit fly and Arabidopsis thaliana . In addition, I reconfirmed that the first introns in humans are spliced slower than those in S. pombe , fruit fly, and Arabidopsis thaliana genome-widely. Both the calculated most likely orders and the method developed here are available on the web. Conclusions A novel computational method was developed to calculate the intron splicing orders and applied the method to real sequencing datasets. I obtained intron splicing orders for hundreds or thousands of genes in four organisms. I found humans contain more number of not in-order spliced transcripts.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Algorithms / Alternative splicing / Arabidopsis thaliana / Bayesian network / Bioinformatics / Biomedical and Life Sciences / Computational Biology/Bioinformatics / Computer Appl. in Life Sciences / Computer applications / Data analysis / Datasets / Fruit flies / Fruit flies (Tephritidae) / Fruits / Genes / Genomes / Genomics / Intron splicing order / Introns / Laws, regulations and rules / Life Sciences / Mathematical analysis / Methodology / Methodology Article / Microarrays / Most likely order / Novel computational methods for the analysis of biological systems / Simulation / Splicing / Transcription / Web sites (World Wide Web)