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Multiplex Cytological Profiling Assay to Measure Diverse Cellular States

by Ljosa, Vebjorn , Carrel, Hyman A. , Shamji, Alykhan F. , Sokolnicki, Katherine L. , Golub, Todd R. , Kemp, Melissa M. , Gustafsdottir, Sigrun M. , Walpita, Deepika , Anthony Wilson, J. , Schreiber, Stuart L. , Clemons, Paul A. , Carpenter, Anne E. , Petri Seiler, Kathleen

in Assaying / Bioactive compounds / Bioassay / Cell Line, Tumor / Cells (Biology) / Chemical compounds / Computer applications / Fluorescence / Fluorescent Dyes - chemistry / Fluorescent indicators / Gene expression / Humans / Image Processing, Computer-Assisted / Informatics / Ligands / Microscopy / Mitochondria / Morphology / Multiplexing / Physiological aspects / Protein structure

2013

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2013

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2013

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Journal Article

Multiplex Cytological Profiling Assay to Measure Diverse Cellular States

Ljosa, Vebjorn,

Carrel, Hyman A.,

Shamji, Alykhan F.,

Sokolnicki, Katherine L.,

Golub, Todd R.,

Kemp, Melissa M.,

Gustafsdottir, Sigrun M.,

Walpita, Deepika,

Anthony Wilson, J.,

Schreiber, Stuart L.,

Clemons, Paul A.,

Carpenter, Anne E.,

Petri Seiler, Kathleen

2013

Overview

Computational methods for image-based profiling are under active development, but their success hinges on assays that can capture a wide range of phenotypes. We have developed a multiplex cytological profiling assay that \"paints the cell\" with as many fluorescent markers as possible without compromising our ability to extract rich, quantitative profiles in high throughput. The assay detects seven major cellular components. In a pilot screen of bioactive compounds, the assay detected a range of cellular phenotypes and it clustered compounds with similar annotated protein targets or chemical structure based on cytological profiles. The results demonstrate that the assay captures subtle patterns in the combination of morphological labels, thereby detecting the effects of chemical compounds even though their targets are not stained directly. This image-based assay provides an unbiased approach to characterize compound- and disease-associated cell states to support future probe discovery.

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Publisher

Public Library of Science,Public Library of Science (PLoS)

Subject

Assaying

/ Bioactive compounds

/ Computer applications