Journal Article

Identification and validation of a m7G-related lncRNA signature for predicting the prognosis and therapy response in hepatocellular carcinoma

Liao, Hui, Dong, Ya-Fang, Peng, Yue-Ling, Guo, Li-Li, Li, Mu-Ye, Li, Rong-Shan

2023

Overview

N7-methylguanosine (m7G) is one of the most common RNA posttranscriptional modifications; however, its potential role in hepatocellular carcinoma (HCC) remains unknown. We developed a prediction signature based on m7G-related long noncoding RNAs (lncRNAs) to predict HCC prognosis and provide a reference for immunotherapy and chemotherapy. RNA-seq data from The Cancer Genome Atlas (TCGA) database and relevant clinical data were used. Univariate and multivariate Cox regression analyses were conducted to identify m7G-related lncRNAs with prognostic value to build a predictive signature. We evaluated the prognostic value and clinical relevance of this signature and explored the correlation between the predictive signature and the chemotherapy treatment response of HCC. Moreover, an in vitro study to validate the function of CASC19 was performed. Six m7G-related lncRNAs were identified to create a signature. This signature was considered an independent risk factor for the prognosis of patients with HCC. TIDE analyses showed that the high-risk group might be more sensitive to immunotherapy. ssGSEA indicated that the predictive signature was strongly related to the immune activities of HCC. HCC in high-risk patients was more sensitive to the common chemotherapy drugs bleomycin, doxorubicin, gemcitabine, and lenalidomide. In vitro knockdown of CASC19 inhibited the proliferation, migration and invasion of HCC cells. We established a 6 m7G-related lncRNA signature that may assist in predicting the prognosis and response to chemotherapy and immunotherapy of HCC.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Accuracy / Analysis / Biology and life sciences / Bleomycin / Calibration / Cancer / Cancer therapies / Carcinoma, Hepatocellular - drug therapy / Carcinoma, Hepatocellular - genetics / Care and treatment / Cell proliferation / Chemotherapy / Datasets / Doxorubicin / Gastric cancer / Gemcitabine / Gene expression / Genetic aspects / Genomes / Genomics / Health risks / Hepatocellular carcinoma / Hepatoma / Humans / Immune response / Immunosuppressive agents / Immunotherapy / Liver cancer / Liver Neoplasms - drug therapy / Liver Neoplasms - genetics / Medical prognosis / Medicine and Health Sciences / Non-coding RNA / Patients / Post-transcription / Probability / Prognosis / Regression analysis / Risk factors / Risk groups / RNA / RNA, Long Noncoding - genetics / Software / Targeted cancer therapy / Transfer RNA