Journal Article

Full-field optical coherence tomography for the diagnosis of giant cell arteritis

Martin, Laurent, Benoit, Emilie, Creuzot-Garcher, Catherine, Boccara, Claude, Bonnotte, Bernard, Greigert, Hélène, Gabrielle, Pierre-Henry, Maldiney, Thomas, Chassot, Jean-Marie, Audia, Sylvain, Tavitian, Bertrand, Samson, Maxime, Balvay, Daniel, Clément, Olivier

2020

Overview

Histopathological examination of temporal artery biopsy (TAB) remains the gold standard for the diagnosis of giant cell arteritis (GCA) but is associated with essential limitations that emphasize the need for an upgraded pathological process. This study pioneered the use of full-field optical coherence tomography (FF-OCT) for rapid and automated on-site pathological diagnosis of GCA. Sixteen TABs (12 negative and 4 positive for GCA) were selected according to major histopathological criteria of GCA following hematoxylin-eosin-saffron-staining for subsequent acquisition with FF-OCT to compare structural modifications of the artery cell wall and thickness of each tunica. Gabor filtering of FF-OCT images was then used to compute TAB orientation maps and validate a potential automated analysis of TAB sections. FF-OCT allowed both qualitative and quantitative visualization of the main structures of the temporal artery wall, from the internal elastic lamina to the vasa vasorum and red blood cells, unveiling a significant correlation with conventional histology. FF-OCT imaging of GCA TABs revealed destruction of the media with distinct remodeling of the whole arterial wall into a denser reticular fibrous neo-intima, which is distinctive of GCA pathogenesis and accessible through automated Gabor filtering. Rapid on-site FF-OCT TAB acquisition makes it possible to identify some characteristic pathological lesions of GCA within a few minutes, paving the way for potential machine intelligence-based or even non-invasive diagnosis of GCA.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Aged / Aged, 80 and over / Arteritis / Autoimmune diseases / Biology and Life Sciences / Biopsy / Blood cells / Case-Control Studies / Cell walls / Cohort Studies / Computer and Information Sciences / Diagnosis / Engineering and Technology / Erythrocytes / Female / Giant Cell Arteritis - diagnosis / Giant Cell Arteritis - diagnostic imaging / Giant Cell Arteritis - pathology / Histology / Hospitals / Human health and pathology / Humans / Hyperplasia / Image filters / Immunology / Intelligence / Internal medicine / Life Sciences / Male / Medical diagnosis / Medicine / Medicine and Health Sciences / Microscopy / Middle Aged / Optical Coherence Tomography / Optical tomography / Pathogenesis / Pathology / Prospective Studies / Qualitative analysis / Research and Analysis Methods / Sensory Organs / Temporal Arteries - diagnostic imaging / Temporal Arteries - pathology / Temporal arteritis / Tomography / Tomography, Optical Coherence - methods / Tunica Intima - diagnostic imaging / Tunica Intima - pathology / Tunica Media - diagnostic imaging / Tunica Media - pathology / Vein & artery diseases / Veins & arteries