Journal Article

Antimicrobial susceptibility and molecular characteristics of Mycoplasma pneumoniae isolates across different regions of China

Zhao, Fei, Guan, Xuemei, He, Lihua, Liu, Jinrong, Gong, Jie, Li, Jing, Zhang, Jianzhong, Meng, Fanliang, Liu, Liyong

2019

Overview

Background In China mainland, most Mycoplasma pneumoniae related studies are carried out in Beijing and Shanghai, while rare studies are performed in the other regions. In this study, we analyzed the molecular biology characteristics and antimicrobial susceptibility of clinical isolates of M. pneumoniae from 5 regions between January 2017 and December 2018. Methods Genotyping was performed to 154  M. pneumoniae isolates from 5 cities using PCR and multiple-locus variable-number tandem repeat analysis (MLVA) method. Antimicrobial susceptibility test was performed to all the isolates against 4 antibiotics. Sequencing was performed to the amplification products of the 23S rRNA drug resistant gene. Results Genotype I was detected in 118  M. pneumoniae isolates (76.6%), and genotype II was identified in 36 isolates (23.4%). The majority (92.2%) of the MLVA genotypes were 4–5–7-2 and 3–5–6-2, which represented the genotype I and II, respectively. The total macrolide (ML) resistance rate was 79.7%. The minimum inhibitory concentration (MIC) of the erythromycin was in a range of 128- > 256 μg/ml, while that for the azithromycin was 2-32 μg/ml. There were mutations in the 23S rRNA in each ML resistance isolate. Jilin city showed the highest prevalence of genotype I (100%) and ML resistance rate (100%), while Jinan showed the lowest prevalence of genotype I (45.5%) and ML resistance rate (54.5%). Conclusions A large variance was identified in the M. pneumoniae genotype and ML resistance among the 5 cities. The proportion of M. pneumoniae with a genotype II genotype (3–5–6-2) showed an increased trend.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: 23S rRNA gene / Antibacterial agents / Antibiotics / Antimicrobial agents / Azithromycin / Azithromycin - pharmacology / Backup software / Biomedical and Life Sciences / Biomedicine / China - epidemiology / Cities and towns / DNA, Ribosomal - genetics / Drug Resistance / Drug Resistance, Bacterial / Erythromycin / Erythromycin - pharmacology / Genes / Genotype / Genotype & phenotype / Genotypes / Genotyping Techniques - methods / Humans / Infectious Diseases / Macrolide resistance / Medical Microbiology / Medical research / Microbial Sensitivity Tests / Minisatellite Repeats / Molecular biology / Multilocus Sequence Typing / Mycoplasma pneumoniae / Mycoplasma pneumoniae - drug effects / Mycoplasma pneumoniae - genetics / Mycoplasma pneumoniae - isolation & purification / Pneumonia / Pneumonia, Mycoplasma - microbiology / Respiratory tract infections / Risk factors / RNA / RNA, Ribosomal, 23S - genetics / Sequence Analysis, DNA / Tetracyclines