Journal Article

Nutritional, molecular, and functional properties of a novel enzymatically hydrolyzed porcine plasma product

Benavente, Fernando, Miró, Lluïsa, Bellver-Sanchis, Aina, Griñán-Ferré, Christian, Polo, Javier, Moretó, Miquel, Barbosa, José, Rodríguez, Carmen, Solà-Ginés, Marc, Pallàs, Mercè, Pont, Laura, Pérez-Bosque, Anna

2024

Overview

In the present study, an enzymatically hydrolyzed porcine plasma (EHPP) was nutritionally and molecularly characterized. EHPP molecular characterization showed, in contrast to spray-dried plasma (SDP), many peptides with relative molecular masses (M r ) below 8,000, constituting 73% of the protein relative abundance. IIAPPER, a well-known bioactive peptide with anti-inflammatory and antioxidant properties, was identified. In vivo functionality of EHPP was tested in C . elegans and two different mouse models of intestinal inflammation. In C . elegans subjected to lipopolysaccharide exposure, EHPP displayed a substantial anti-inflammatory effect, enhancing survival and motility by 40% and 21.5%, respectively. Similarly, in mice challenged with Staphylococcus aureus enterotoxin B or Escherichia coli O42, EHPP and SDP supplementation (8%) increased body weight and average daily gain while reducing the percentage of regulatory Th lymphocytes. Furthermore, both products mitigated the increase of pro-inflammatory cytokines expression associated with these challenged mouse models. In contrast, some significant differences were observed in markers such as Il-6 and Tnf-α , suggesting that the products may present different action mechanisms. In conclusion, EHPP demonstrated similar beneficial health effects to SDP, potentially attributable to the immunomodulatory and antioxidant activity of its characteristic low M r bioactive peptides.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Amino acids / Animal models / Animals / Anti-Inflammatory Agents - pharmacology / Antioxidants / Antioxidants - metabolism / Biological activity / Biological products / Body weight / Caenorhabditis elegans - metabolism / Chromatography / Cytokines / Cytokines - metabolism / E coli / Escherichia coli / Feeds / Food / Hydrolysis / Immune response / Immunomodulation / Inflammation / Lipopolysaccharides / Lymphocytes / Mass spectrometry / Mice / Peptides / Plasma / Plasma - metabolism / Proteins / Relative abundance / Scientific equipment and supplies industry / Scientific imaging / Staphylococcal enterotoxin B / Swine