Journal Article

Selected prebiotics and synbiotics administered in ovo can modify innate immunity in chicken broilers

Stefaniak, Tadeusz, Bednarczyk, Marek, Siwek, Maria, Łukaszewicz, Ewa, Sieńczyk, Marcin, Kowalczyk, Artur, Madej, Jan P., Graczyk, Stanisław

2019

Overview

Background A previous study showed that prebiotics and synbiotics administered in ovo into the egg air cell on the 12th day of incubation enhance the growth and development of chickens. However, the influence of this procedure on the development and efficiency of the innate immune system of broiler chickens is unclear. Therefore, the aim of this study was to evaluate whether the early (on the 12th day of embryo development) in ovo administration of selected prebiotics (inulin − Pre1 and Bi 2 tos − Pre2) and synbiotics (inulin + Lactococcus lactis subsp. lactis IBB SL1 − Syn1 and Bi 2 tos +  L. lactis subsp. cremoris IBB SC1 − Syn2) influences the innate immune system. Results Chickens (broiler, Ross 308) that were treated with Pre1 exhibited a decreased H/L ratio on D7, but an increased H/L ratio was observed on D21 and D35. In the remaining experimental groups, an increase in the H/L ratio was observed on D21 and D35. The oxidative potential of leukocytes measured using the NBT test increased on D21 in Pre2 and Syn1 groups. The rate of the phagocytic ability of leukocytes increased in Pre1 and Syn1 groups on D21. The phagocytic index decreased in Pre1 and Syn2 groups on D21 and D35. Concurrently, the count of WBC in circulating blood decreased on D21 in Pre1, Pre2, and Syn1 groups. The hematocrit value was increased in Syn1 chickens on D21, in Pre1 chickens on D35, and in Syn2 chickens on both time points. Conclusions Early in ovo treatment of chicken embryos with prebiotics and synbiotics may temporarily modulate not only the production/maturation of leukocytes but also their reactivity.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: air / Animal embryo / Animals / Bacteria / Blood Proteins - analysis / broiler chickens / Broilers (Chickens) / Care and treatment / Chick Embryo - drug effects / Chick Embryo - immunology / chick embryos / Chicken / Chickens - blood / Chickens - immunology / Clinical pathology / Dosage and administration / Eggs / Eggs (Food) / Embryo / embryogenesis / Embryonic development / Embryos / Gene expression / Health aspects / Hematocrit / Hematocrit - veterinary / Hematology / Immune system / Immunity, Innate - drug effects / Immunology / In ovo / Innate immunity / Inulin / Lactococcus lactis / Lactococcus lactis subsp. cremoris / Lactococcus lactis subsp. lactis / Leukocyte Count - veterinary / Leukocytes / Lymphocytes / Medicine / Medicine & Public Health / Microbiota (Symbiotic organisms) / Nutrition / Phagocytes / phagocytosis / Phagocytosis - drug effects / Physiological aspects / physiology and immunology / Poultry / Prebiotic / Prebiotics / Prebiotics - administration & dosage / Probiotics / Research Article / Salmonella / Synbiotic / Synbiotics - administration & dosage / Transgenics / Veterinary dietary supplements / veterinary medicine / Veterinary Medicine/Veterinary Science / White blood cell count / Zoology