Journal Article

Generation of full-length circular RNA libraries for Oxford Nanopore long-read sequencing

Babin, Loélia, Fuchs, Steffen, Willier, Semjon, Gaspin, Christine, Andraos, Elissa, Schulte, Johannes H., Bessiere, Chloé, Meggetto, Fabienne

2022

Overview

Circular RNA (circRNA) is a noncoding RNA class with important implications for gene expression regulation, mostly by interaction with other RNA species or RNA-binding proteins. While the commonly applied short-read Illumina RNA-sequencing techniques can be used to detect circRNAs, their full sequence is not revealed. However, the complete sequence information is needed to analyze potential interactions and thus the mechanism of action of circRNAs. Here, we present an improved protocol to enrich and sequence full-length circRNAs by using the Oxford Nanopore long-read sequencing platform. The protocol involves an enrichment of lowly abundant circRNAs by exonuclease treatment and negative selection of linear RNAs. Then, a cDNA library is created and amplified by PCR. This protocol provides enough material for several sequencing runs. The library is used as input for ligation-based sequencing together with native barcoding. Stringent quality control of the libraries is ensured by a combination of Qubit, Fragment Analyzer and qRT-PCR. Multiplexing of up to 4 libraries yields in total more than 1–2 Million reads per library, of which 1–2% are circRNA-specific reads with >99% of them full-length. The protocol works well with human cancer cell lines. We further provide suggestions for the bioinformatic analysis of the created data, as well as the limitations of our approach together with recommendations for troubleshooting and interpretation. Taken together, this protocol enables reliable full-length analysis of circRNAs, a noncoding RNA type involved in a growing number of physiologic and pathologic conditions. Metadata Associated content . https://dx.doi.org/10.17504/protocols.io.rm7vzy8r4lx1/v2 .

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Analysis / Binding proteins / Biochemistry, Molecular Biology / Bioinformatics / Biology and life sciences / Biotechnology / Circular RNA / Exonuclease / Gene expression / Gene regulation / Gene sequencing / Genomics / Lab Protocol / Life Sciences / Lymphoma / Multiplexing / Negative selection / Nucleotide sequence / Protocol / Quality control / Quantitative Methods / Qubits (quantum computing) / Research and analysis methods / Ribonucleic acid / RNA / RNA-binding protein / Troubleshooting / Tumor cell lines