Journal Article

Quorum sensing in thermophiles: prevalence of autoinducer-2 system

Capalash, Neena, Sharma, Prince, Kaur, Amandeep

2018

Overview

Background Quorum sensing is a mechanism of cell to cell communication that requires the production and detection of signaling molecules called autoinducers. Although mesophilic bacteria is known to utilize this for synchronization of physiological processes such as bioluminescence, virulence, biofilm formation, motility and cell competency through signaling molecules (acyl homoserine lactones, AI-1; oligopeptides, peptide based system and furanosyl borate diester, AI-2), the phenomenon of quorum sensing in thermophiles is largely unknown. Results In this study, proteomes of 106 thermophilic eubacteria and 21 thermophilic archaea have been investigated for the above three major quorum sensing systems to find the existence of quorum sensing in these thermophiles as there are evidences for the formation of biofilms in hot environments. Our investigation demonstrated that AI-1 system is absent in thermophiles. Further, complete peptide based two component systems for quorum sensing was also not found in any thermophile however the traces for the presence of response regulators for peptide based system were found in some of them. BLASTp search using LuxS (AI-2 synthase) protein sequence of Escherichia coli str. K-12 substr. MG1655 and autoinducer-2 receptors (LuxP of Vibrio harveyi , LsrB of E. coli str. K-12 substr. MG1655 and RbsB of Aggregatibacter actinomycetemcomitans ) as queries revealed that 17 thermophilic bacteria from phyla Deinococcus- Thermus and Firmicutes possess complete AI-2 system (LuxS and LsrB and/or RbsB). Out of 106 thermophilic eubacteria 18 from phyla Deinococcus- Thermus , Proteobacteria and Firmicutes have only LuxS that might function as AI-2 synthesizing protein whereas, 16 are having only LsrB and/or RbsB which may function as AI-2 receptor in biofilms. Conclusions We anticipate that thermophilic bacteria may use elements of LsrB and RbsB operon for AI-2 signal transduction and they may use quorum sensing for purposes like biofilm formation. Nevertheless, thermophiles in which no known quorum sensing system was found may use some unknown mechanisms as the mode of communication. Further information regarding quorum sensing will be explored to develop strategies to disrupt the biofilms of thermophiles.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Aggregatibacter actinomycetemcomitans / Amino acid sequence / Archaea / Archaea - classification / Archaea - genetics / Archaea - metabolism / Archaeal Proteins - genetics / Archaeal Proteins - metabolism / Autoinducer-2 / Bacteria / Bacteria - classification / Bacteria - genetics / Bacteria - metabolism / Bacteria, Thermophilic / Bacterial Proteins - genetics / Bacterial Proteins - metabolism / Biofilms / Biofilms - growth & development / Biological Microscopy / Bioluminescence / Biomedical and Life Sciences / Cell interaction / Cell interactions / Cellular signal transduction / Chemical communication / Communication / Communications systems / Deinococcus / Detection / E coli / Escherichia coli / Firmicutes / genomics and proteomics / Homocysteine / Homoserine - analogs & derivatives / Homoserine - metabolism / Homoserine lactones / Hot Temperature / Kinases / Lactones / Lactones - metabolism / Life Sciences / LsrB / LuxS / LuxS protein / Lyme disease / Mesophilic bacteria / Metabolism / Microbial genetics / Microbiology / Molecular chains / Mycology / N-Acyl homoserine lactone / Oligopeptides / Parasitology / Peptides / Phylogeny / Prevalence studies (Epidemiology) / Proteins / Proteobacteria / Proteomics / Pseudomonas aeruginosa / Quorum Sensing / RbsB / Receptors / Regulators / Research Article / Signal Transduction / Signaling / Synchronism / Synchronization / Thermophiles / Thermophilic archaea / Thermophilic bacteria / Vibrio harveyi / Virology / Virulence / Waterborne diseases