Journal Article

Rumen methanogen and protozoal communities of Tibetan sheep and Gansu Alpine Finewool sheep grazing on the Qinghai–Tibetan Plateau, China

Huang, Jinqiang, Li, Yongjuan

2018

Overview

Background Tibetan sheep (TS) and Gansu Alpine Finewool sheep (GS) are both important plateau sheep raised and fed on the harsh Qinghai–Tibetan Plateau, China. Rumen methanogen and protozoal communities of plateau sheep are affected by their hosts and living environments, and play important roles in ruminant nutrition and greenhouse gas production. However, the characteristics, differences, and associations of these communities remain largely uncharacterized. Results The rumen methanogen and protozoal communities of plateau sheep were investigated by 16S/18S rRNA gene clone libraries. The predominant methanogen order in both sheep species was Methanobacteriales followed by Methanomassiliicoccales, which is consistent with those seen in global ruminants. However, the most dominant species was Methanobrevibacter millerae rather than Methanobrevibacter gottschalkii seen in most ruminants. Compared with GS and other ruminants, TS have more exclusive operational taxonomic units and a lower proportion (64.5%) of Methanobrevibacter . The protozoa were divided into Entodiniomorphida and Vestibuliferida, including nine genera and 15 species. The proportion of holotrich protozoa was much lower (1.1%) in TS than ordinary sheep. The most predominant genus was Entodinium (70.0%) in TS and Enoploplastron (48.8%) in GS, while the most common species was Entodinium furca monolobum (43.9%) and Enoploplastron triloricatum (45.0%) in TS and GS, respectively; Entodinium longinucleatum (22.8%) was only observed in TS. LIBSHUFF analysis indicated that the methanogen communities of TS were significantly different from those of GS, but no significant differences were found in protozoal communities. Conclusion Plateau sheep have coevolved with unique rumen methanogen and protozoal communities to adapt to harsh plateau environments. Moreover, the host appears to have a greater influence on rumen methanogen communities than on rumen protozoal communities. The observed associations of methanogens and protozoa, together with the findings of previous studies on methane emissions from ruminant livestock, revealed that the lower proportion of Methanobrevibacter and holotrich protozoa may be responsible for the lower methane emission of TS. These findings facilitate our understanding of the rumen microbial ecosystem in plateau sheep, and could help the development of new strategies to manipulate rumen microbes to improve productivity and reduce the emission of greenhouse gases.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Animals / Biological Microscopy / Biomedical and Life Sciences / Cattle / Communities / Community structure / Dominant species / Ecosystems / Emissions / Emissions control / Environmental aspects / Euryarchaeota - classification / Euryarchaeota - genetics / Euryarchaeota - isolation & purification / Euryarchaeota - metabolism / Feeding Behavior / Feeds / Fermentation / Gas production / Gene expression / Genera / Genomes / Genomics / Greenhouse effect / Greenhouse gases / Health aspects / Host-parasite relationships / Life Sciences / Livestock / Metabolism / Methane / Methane - metabolism / Methanobrevibacter - classification / Methanobrevibacter - genetics / Methanobrevibacter - isolation & purification / Methanobrevibacter - metabolism / Methanogen / Methanogenic bacteria / Methanogens / Microbe-host interactions and microbial pathogenicity / Microbial colonies / Microbiology / Microorganisms / Mycology / Nutrition / Oil and gas production / Parasitology / Phylogeny / Physiological aspects / Plateau sheep / Protozoa / Protozoan diseases / Research Article / Risk factors / rRNA 16S / rRNA 18S / Rumen / Rumen - microbiology / Rumen - parasitology / Rumen protozoa / Ruminant nutrition / Sheep / Sheep - microbiology / Sheep - parasitology / Sheep - physiology / Tibet / Trichostomatida - genetics / Trichostomatida - isolation & purification / Trichostomatida - metabolism / Virology