Journal Article

Na+/K+ Balance and Transport Regulatory Mechanisms in Weedy and Cultivated Rice (Oryza sativa L.) Under Salt Stress

Fang, Jiapeng, Zhang, Yuhua, Dong, Liyao, Wu, Xibao

2018

Overview

Background Salinization is a primary abiotic stress constraining global plant growth and production. Weedy rice, though highly homologous to cultivated rice, is more salt tolerant during seed germination and seedling growth; we hypothesize that this is owing to ionic homeostasis and changes in the expression of genes encoding ion transport regulators. Results The four different genotypes of weedy ( JYGY-1 and JYFN-4 ) and cultivated ( Nipponbare and 9311 ) rice have different salt-tolerance during seed germination and seedling vegetative growth under salt stress. In this study, Na + and Ca 2+ content increased in weedy and cultivated rice genotypes under salt stress while K + and Mg 2+ decreased; however, JYGY-1 had the lowest Na + /K + ratio of assessed genotypes. Genes in the high-affinity K + transporter ( HKT ) and tonoplast sodium-hydrogen exchanger ( NHX ) families, and salt overly sensitive 1 ( OsSOS1 ) have more than 98% homology in amino acid sequences between weedy and cultivated rice genotypes. Under salt stress, the HKT family members were differentially expressed in the roots and shoots of four different genotypes. However, the NHX family transcripts were markedly up-regulated in all genotypes, but there are significant differences between different genotypes. OsSOS1 was significantly up-regulated in roots, especially in JYGY-1 genotype. Conclusions The results showed that different genotypes had different germination and nutrient survival under salt stress, which was related to the difference of ion content and the difference of a series of ion transport gene expression. At the same time this study will provide new insight into the similarities and differences in ion homeostasis and gene regulatory mechanisms between weedy and cultivated rice under salt stress, which can aid in novel rice breeding and growth strategies.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Abiotic stress / Agriculture / amino acid sequences / Amino acids / Analysis / Biochemistry and physiology / Biomedical and Life Sciences / breeding / Calcium - metabolism / Crop production / Cultivation / Cytoplasm / Evolution / Gene expression / gene expression regulation / Gene Expression Regulation, Plant / Genes / Genes, Plant - genetics / Genes, Plant - physiology / Genetic aspects / Genomes / genotype / Genotypes / Germination / Grain cultivation / Growth / HKT family / Homeostasis / Homology / Ion transport / Ions / Life Sciences / Magnesium - metabolism / messenger RNA / Metabolism / Na+/H+-exchanging ATPase / Na+/K+ homeostasis / NaCl stress / NHX family / Oryza - genetics / Oryza - metabolism / Oryza - physiology / Oryza sativa / Physiological aspects / Plant breeding / Plant growth / Plant Roots - metabolism / Plant Sciences / Plant Shoots - metabolism / Potassium / Potassium - metabolism / Regulators / Regulatory mechanisms (biology) / Research Article / Rice / Roots / Salinity / Salinization / Salt / Salt Stress / Salt stress (Botany) / salt tolerance / Salt-Tolerant Plants - genetics / Salts / Seed germination / seedling growth / Seedlings / Seedlings - growth & development / Sequence Homology / Sequences / Shoots / Sodium / Sodium - metabolism / sodium-hydrogen antiporter / Sodium-Potassium-Exchanging ATPase - genetics / Sodium-Potassium-Exchanging ATPase - metabolism / Sodium-Potassium-Exchanging ATPase - physiology / tonoplast / Tree Biology / Vacuolar SOS1 / vegetative growth / Weeds / Weedy rice