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Molecular characterization and antiviral effects of canine interferon regulatory factor 1 (CaIRF1)
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Molecular characterization and antiviral effects of canine interferon regulatory factor 1 (CaIRF1)
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Journal Article
Molecular characterization and antiviral effects of canine interferon regulatory factor 1 (CaIRF1)
2022
Overview
Background
Interferon regulatory factor 1 (IRF1) is an important transcription factor that activates the type I interferon (IFN-I) response and plays a vital role in the antiviral immune response. Although IRF1 has been identified in several mammals, little information related to its function in canines has been described.
Results
In this study, canine IRF1 (CaIRF1) was cloned. After a series of bioinformatics analyses, we found that the CaIRF1 protein structure was similar to that of other animal IRF1 proteins, including a conserved DNA-binding domain (DBD), an IRF-association domain 2 (IAD2) domain and two nuclear localization signals (NLSs). An indirect immunofluorescence assay (IFA) revealed that CaIRF1 was mainly distributed in the nucleus. Overexpression of CaIRF1 in Madin-Darby canine kidney cells (MDCK) induced high levels of interferon β (IFNβ) and IFN-stimulated response element (ISRE) promoter activation and induced interferon-stimulated gene (ISG) expression. Subsequently, we assayed the antiviral activity of CaIRF1 against vesicular stomatitis virus (VSV) and canine parvovirus type-2 (CPV-2) in MDCK cells. Overexpression of CaIRF1 effectively inhibited the viral yields of VSV and CPV-2, while knocking down of CaIRF1 expression mildly increased viral gene copies.
Conclusions
CaIRF1 is involved in the cellular IFN-I signaling pathway and plays an important role in the antiviral response.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Animals
/ Antiviral Agents - pharmacology
/ Canine interferon regulatory factor 1
/ Cloning
/ CPV-2
/ Dogs
/ domain
/ fluorescent antibody technique
/ genes
/ Interferon regulatory factor
/ Interferon regulatory factor 1
/ Interferon Regulatory Factor-1 - genetics
/ Interferon Regulatory Factor-1 - metabolism
/ ISGs
/ ISRE
/ kidneys
/ Mammals
/ Medicine
/ Proteins
/ Testing
/ Veterinary Medicine/Veterinary Science
/ Viruses
/ Zoology
