Journal Article

Screening for proteins related to the biosynthesis of hispidin and its derivatives in Phellinus igniarius using iTRAQ proteomic analysis

Zhou, Li, Li, Yuanjie, Guo, Jinjing, Huang, Yu, Jiang, Zhibo, Bai, Changcai, Liu, Xiaoxi, Liu, Cheng, Wu, Xiuli, Ji, Hongyan

2021

Overview

Background Hispidin (HIP) and its derivatives, a class of natural fungal metabolites, possess complex chemical structures with extensive pharmacological activities. Phellinus igniarius , the most common source of HIP, can be used as both medicine and food. However, the biosynthetic pathway of HIP in P. igniarius remains unclear and we have a limited understanding of the regulatory mechanisms related to HIP. In this work, we sought to illustrate a biosynthesis system for hispidin and its derivatives at the protein level. Results We found that tricetolatone (TL) is a key biosynthetic precursor in the biosynthetic pathway of hispidin and that its addition led to increased production of hispidin and various hispidin derivatives. Based on the changes in the concentrations of precursors and intermediates, key timepoints in the biosynthetic process were identified. We used isobaric tags for relative and absolute quantification (iTRAQ) to study dynamic changes of related proteins in vitro. The 270 differentially expressed proteins were determined by GO enrichment analysis to be primarily related to energy metabolism, oxidative phosphorylation, and environmental stress responses after TL supplementation. The differentially expressed proteins were related to ATP synthase, NAD binding protein, oxidoreductase, and other elements associated with electron transfer and dehydrogenation reactions during the biosynthesis of hispidin and its derivatives. Multiple reaction monitoring (MRM) technology was used to selectively verify the iTRAQ results, leading us to screen 11 proteins that were predicted to be related to the biosynthesis pathways. Conclution These findings help to clarify the molecular mechanism of biosynthesis of hispidin and its derivatives and may serve as a foundation for future strategies to identify new hispidin derivatives.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: ATP synthase / Biological Microscopy / Biomedical and Life Sciences / Biosynthesis / Cellular signal transduction / Chemical properties / Dehydrogenation / Dietary supplements / Electron transfer / Energy metabolism / Environmental stress / Fermentation / Fungi / Gene expression / Hispidin / Intermediates / iTRAQ / Life Sciences / Metabolism / Metabolites / Methods / Microbial biochemistry / Microbiological research / Microbiological synthesis / Microbiology / Molecular weight / Mycology / NAD / Oxidative metabolism / Oxidative phosphorylation / Oxidoreductase / Parasitology / Peptides / Phellinus igniarius / Phosphorylation / Physiological aspects / physiology and metabolism / Polyphenols / Precursors / Proteins / Proteomic / Proteomics / Regulatory mechanisms (biology) / Research Article / Virology