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Local slowdown of translation by nonoptimal codons promotes nascent-chain recognition by SRP in vivo
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Local slowdown of translation by nonoptimal codons promotes nascent-chain recognition by SRP in vivo
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Local slowdown of translation by nonoptimal codons promotes nascent-chain recognition by SRP in vivo
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Journal Article
Local slowdown of translation by nonoptimal codons promotes nascent-chain recognition by SRP in vivo
2014
Overview
Analyses of yeast codon usage and ribosome profiling data reveal a nonoptimal codon cluster in the mRNAs of ER-targeted proteins, downstream of the SRP-binding site, that would slow down translation to promote SRP interaction.
The genetic code allows most amino acids a choice of optimal and nonoptimal codons. We report that synonymous codon choice is tuned to promote interaction of nascent polypeptides with the signal recognition particle (SRP), which assists in protein translocation across membranes. Cotranslational recognition by the SRP
in vivo
is enhanced when mRNAs contain nonoptimal codon clusters 35–40 codons downstream of the SRP-binding site, the distance that spans the ribosomal polypeptide exit tunnel. A local translation slowdown upon ribosomal exit of SRP-binding elements in mRNAs containing these nonoptimal codon clusters is supported experimentally by ribosome profiling analyses in yeast. Modulation of local elongation rates through codon choice appears to kinetically enhance recognition by ribosome-associated factors. We propose that cotranslational regulation of nascent-chain fate may be a general constraint shaping codon usage in the genome.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
