Journal Article

Pseudomonas ZY-1 and Bacillus FY-1 protecting the rice seedlings from the harm of Pseudomonas aeruginosa via indirect seawead lysis

Zhu, Yuan, Wu, Zhihai, Xu, Yufeng, Yang, Meiying, Zhou, Xueying, Wei, Xiaoshuang, Wang, Jianing, Yang, Xue, Yue, Chengcai, Li, Siyuan, Wu, Lei, Tian, Shiqi

2024

Overview

The local ecosystems, fishery and human health are all threatened by water blooms, so effectively controlling water blooms has become an urgent and challenging issue. Biological control of water blooms is given priority due to its low cost, high efficiency and environmental friendliness. In this study, Pseudomonas ZY-1 and Bacillus FY-1, two highly-effective algicidal bacteria strains which are able to indirectly lyse algae by separating and screening from the vigorous water body in the paddy alga of Northeast China were obtained. The two bacterial strains have stronger ability to lyse alga in the bacterial liquid concentration of 10 6 CFU/ml, and the alga-lysing rate on 7 d reached 84.03% and 83.11% respectively. The active substance secreted by ZY-1 is not sensitive to the changes of temperature and pH value, while as FY-1 cell-free filtrate is not stable in high temperature above 50 ℃ and pH of 5, it requires the sun light to have the algaecidal effect. The cell-free filtrates of strains ZY-1 and FY-1 had the best lysis effect on Microcystis aeruginosa cells , and the chlorophyll a content of algae decreased to 0.13 ± 0.02 mg/L and 0.14 ± 0.03 mg/L respectively and the Fv/Fm of Microcystis aeruginosa decreased by 97.22% after 7 days. The algaecidal process of ZY-1 and FY-1 may be that the cell-free filtrate inhibits the photosynthesis of Microcystis aeruginosa , and meanwhile it avoids the regeneration and repair of photosynthesis of algal cells by affecting the gene expression and damaging the repair system of algal cells, so the membrane lipid peroxidation is exacerbated and then the membrane of algal cells is broken, the algal cells can’t do normal life activities, and finally the algal cell would be killed. The rice seedlings in the algal liquid treatment group are short and show root dysplasia, few roots and brown roots. After treated with cell-free filtrate of ZY-1 and FY-1, the oxidative damage of the rice is obviously reduced, and the harm from Microcystis aeruginosa is reduced, which has the repair effect to the roots of rice seedlings and its aboveground growth. The cell-free filtrate of FY-1 works better than ZY-1. The bacteria strains of ZY-1 and FY-1 have the indirect algaecide trait, which makes them the potential environmentally-friendly algaecidal bacteria and they show broad application in the agricultural production and the control of water blooms.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Agricultural production / Agricultural research / Agriculture / Algae / Algicides / Aquatic ecosystems / Aquatic plants / Bacillus / Bacillus (Bacteria) / Bacillus - genetics / Bacillus - metabolism / Bacillus - physiology / Bacillus FY-1 / Bacteria / Biological control / Biological Control Agents / Biological effects / Biological Microscopy / Biomedical and Life Sciences / Chemical properties / China / Chlorophyll / Chlorophyll A - metabolism / Climate change / Control / Damage / Decomposition (Chemistry) / Diseases and pests / Ecosystems / Environmental aspects / Environmental impact / Filtrate / Fisheries / Gene expression / Genomes / Harmful Algal Bloom / Herbicides / High temperature / Hydrogen-Ion Concentration / Indirect algaecide trai / Life Sciences / Light / Lipid peroxidation / Lipids / Lysis / Marine algae / Membranes / Microbiology / Microcystis / Microcystis - genetics / Microcystis - growth & development / Microcystis - metabolism / Microcystis - physiology / Microcystis aeruginosa / Microorganisms / Mycology / Nitrogen / Nutrition / Oryza - microbiology / Parasitology / Peroxidation / Pests / Phosphorus / Photosynthesis / Production processes / Pseudomonas / Pseudomonas - genetics / Pseudomonas - metabolism / Pseudomonas - physiology / Pseudomonas aeruginosa - genetics / Pseudomonas aeruginosa - metabolism / Pseudomonas aeruginosa - physiology / Pseudomonas aeruginosa infections / Pseudomonas ZY-1 / Rice / Roots / Seedlings / Seedlings - growth & development / Seedlings - microbiology / Sewage disposal / Strains (organisms) / Temperature / Temperature requirements / Virology / Water / Water bodies