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Interaction between basigin and monocarboxylate transporter 2 in the mouse testes and spermatozoa
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Interaction between basigin and monocarboxylate transporter 2 in the mouse testes and spermatozoa
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Journal Article
Interaction between basigin and monocarboxylate transporter 2 in the mouse testes and spermatozoa
2016
Overview
Basigin is a member of the immunoglobulin superfamily and plays various important roles in biological events including spermatogenesis. To examine the basigin molecular variants during spermatogenesis and sperm maturation in the mouse, immunoprecipitated basigin samples from testis and epididymal spermatozoa were analyzed by liquid chromatography-mass spectrometry/mass spectrometry （LC-MS/MS）. The results demonstrated that basigin molecules from the testis and spermatozoa were separable into two major bands and that the differences in the molecular sizes were possibly because of an endoproteolytic cleavage. Since basigin is known to be a chaperone for the monocarboxylate transporter 1 （MCT1）, the localization of basigin, MCT1 and MCT2 was examined during postnatal testicular development. Immunohistochemical studies showed different expression patterns of MCT1 and MCT2. MCT1 was localized on the surface of spermatogonia, spermatocytes, and spermatids. In contrast, MCT2 appeared on the principal piece of spermatozoa in the testis, where basigin was also observed. In mature epididymal spermatozoa, MCT2 was located on the midpiece, where basigin co-localized with MCT2 but not with MCT1. Furthermore, MCT2 was immunoprecipitated with basigin in mouse testes and sperm. These results suggest that basigin has a functional role as a binding partner with MCT2 in testicular and epididymal spermatozoa.
Publisher
Wolters Kluwer - Medknow Publications,Medknow Publications and Media Pvt. Ltd,Medknow Publications & Media Pvt. Ltd,Medknow Publications & Media Pvt Ltd,Wolters Kluwer Medknow Publications
Subject
/ basigin; monocarboxylate transporter; sperm maturation; spermatogenesis; spermatozoa; testis
/ LC-MS/MS
/ Male
/ Mice
/ Monocarboxylic Acid Transporters - metabolism
/ Original
/ Proteins
/ Rodents
/ Sperm
/ Sperm Maturation - physiology
/ Spermatogenesis - physiology
/ Studies
/ 免疫球蛋白超家族
/ 小鼠睾丸
/ 相互作用
/ 精子发生
/ 羧酸
/ 转运蛋白
