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Electrochemical biosensing of galactose based on carbon materials: graphene versus multi-walled carbon nanotubes
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Electrochemical biosensing of galactose based on carbon materials: graphene versus multi-walled carbon nanotubes
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Journal Article
Electrochemical biosensing of galactose based on carbon materials: graphene versus multi-walled carbon nanotubes
2016
Overview
In this study, two enzyme electrodes based on graphene (GR), Co
3
O
4
nanoparticles and chitosan (CS) or multi-walled carbon nanotubes (MWCNTs), Co
3
O
4
nanoparticles, and CS, were fabricated as novel biosensing platforms for galactose determination, and their performances were compared. Galactose oxidase (GaOx) was immobilized onto the electrode surfaces by crosslinking with glutaraldehyde. Optimum working conditions of the biosensors were investigated and the analytical performance of the biosensors was compared with respect to detection limit, linearity, repeatability, and stability. The MWCNTs-based galactose biosensor provided about 1.6-fold higher sensitivity than its graphene counterpart. Moreover, the linear working range and detection limit of the MWCNTs-based galactose biosensor was superior to the graphene-modified biosensor. The successful application of the purposed biosensors for galactose biosensing in human serum samples was also investigated.
Publisher
Springer Berlin Heidelberg,Springer,Springer Nature B.V
Subject
/ Biosensing Techniques - instrumentation
/ Biosensing Techniques - methods
/ Carbon
/ Characterization and Evaluation of Materials
/ Chemistry and Materials Science
/ Chitosan
/ Electrochemical Techniques - instrumentation
/ Electrochemical Techniques - methods
/ Enzymes
/ Enzymes, Immobilized - chemistry
/ Graphene
/ Humans
/ Monitoring/Environmental Analysis
