Asset Details
Do you wish to reserve the book?
Effect of nitrogen supply on nitrogen metabolism in the citrus cultivar ‘Huangguogan’
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Effect of nitrogen supply on nitrogen metabolism in the citrus cultivar ‘Huangguogan’
Do you wish to request the book?
Effect of nitrogen supply on nitrogen metabolism in the citrus cultivar ‘Huangguogan’
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Effect of nitrogen supply on nitrogen metabolism in the citrus cultivar ‘Huangguogan’
2019
Overview
Nitrogen metabolism in citrus has received increased attention due to its effects on plant growth and productivity. However, little is known about the effects of nitrogen fertilization on nitrogen metabolism in young trees of citrus cultivar 'Huangguogan' (Citrus reticulata × Citrus sinensis). Here, genes encoding nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS), glutamate dehydrogenase (GDH), and asparagine synthetase (AS), represented as HgNR, HgNiR, HgGS, HgGDH, and HgAS, respectively, were cloned from Huangguogan. Deduced protein sequences were analyzed and proteins were confirmed to be localized in their respective cellular organelles. Moreover, pot-cultured 'Huangguogan' seedlings were fertilized with 0 (N1), 1.36 (N2), 1.81 (N3), 2.26 (N4), or 2.72 (N5) kg N/year, for 12 months. Enzyme activity and enzyme-gene expression were studied in roots, leaves, and fruits at different stages. Finally, the effects of N application rate on root activity, leaf N, soluble protein, yield, and fruit quality at the ripening stage were measured. The results showed that: 1) HgNR, HgNiR, HgGDH, and HgAS gene products were found mainly in the cytoplasm and plasma membrane, while HgGS gene product was found mainly in cytoplasm and mitochondria. 2) Gene expression and enzyme activity differed among plant organs. As the root is in permanent direct contact with the soil we suggest that root gene expression and enzyme activity can be used as reference to determine N application rate. 3) Yield, fruit quality, enzyme activity, and enzyme-related gene expression were considerably lower at low than at high-N supply. However, they were all inhibited by excess nitrogen (i.e., 2.72 kg/year). Therefore, we recommend 1.81 kg N/year as the optimal N application rate for young 'Huangguogan' trees.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Analysis
/ Citrus
/ Citrus - growth & development
/ Enzymes
/ Fruits
/ Gene Expression Regulation, Plant
/ Genes
/ Glutamate Dehydrogenase - classification
/ Glutamate Dehydrogenase - genetics
/ Glutamate Dehydrogenase - metabolism
/ Glutamate-Ammonia Ligase - classification
/ Glutamate-Ammonia Ligase - genetics
/ Glutamate-Ammonia Ligase - metabolism
/ Growth
/ Leaves
/ Ligases
/ Medicine and Health Sciences
/ Nitrate Reductase - classification
/ Nitrate Reductase - genetics
/ Nitrate Reductase - metabolism
/ Nitrates
/ Nitrogen
/ Organs
/ Plant Proteins - classification
/ Proteins
/ Ripening
/ Seedlings - growth & development
/ Trees
