Journal Article

Probiotic Bifidobacterium breve Induces IL-10-Producing Tr1 Cells in the Colon

Ueda, Yoshiyasu, Takahashi, Takuya, Hara, Hiromitsu, Asahara, Takashi, Okumura, Ryu, Nomoto, Koji, Ma, Ji Su, Yamamoto, Masahiro, Kiyono, Hiroshi, Yoshida, Hiroki, Jeon, Seong Gyu, Tsuji, Noriko M., Takeda, Kiyoshi, Tsuji, Hirokazu, Kusu, Takashi, Kayama, Hisako

2012

Overview

Specific intestinal microbiota has been shown to induce Foxp3(+) regulatory T cell development. However, it remains unclear how development of another regulatory T cell subset, Tr1 cells, is regulated in the intestine. Here, we analyzed the role of two probiotic strains of intestinal bacteria, Lactobacillus casei and Bifidobacterium breve in T cell development in the intestine. B. breve, but not L. casei, induced development of IL-10-producing Tr1 cells that express cMaf, IL-21, and Ahr in the large intestine. Intestinal CD103(+) dendritic cells (DCs) mediated B. breve-induced development of IL-10-producing T cells. CD103(+) DCs from Il10(-/-), Tlr2(-/-), and Myd88(-/-) mice showed defective B. breve-induced Tr1 cell development. B. breve-treated CD103(+) DCs failed to induce IL-10 production from co-cultured Il27ra(-/-) T cells. B. breve treatment of Tlr2(-/-) mice did not increase IL-10-producing T cells in the colonic lamina propria. Thus, B. breve activates intestinal CD103(+) DCs to produce IL-10 and IL-27 via the TLR2/MyD88 pathway thereby inducing IL-10-producing Tr1 cells in the large intestine. Oral B. breve administration ameliorated colitis in immunocompromised mice given naïve CD4(+) T cells from wild-type mice, but not Il10(-/-) mice. These findings demonstrate that B. breve prevents intestinal inflammation through the induction of intestinal IL-10-producing Tr1 cells.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Adoptive Transfer / Animals / Bacteria / Bifidobacteriales Infections - immunology / Bifidobacteriales Infections - microbiology / Bifidobacteriales Infections - therapy / Bifidobacterium / Bifidobacterium - immunology / CD4-Positive T-Lymphocytes - immunology / CD4-Positive T-Lymphocytes - transplantation / Cell Differentiation / Coculture Techniques / Colitis - immunology / Colitis - microbiology / Colitis - therapy / Colon (Anatomy) / Colon - immunology / Colon - microbiology / Dendritic Cells - cytology / Dendritic Cells - immunology / Dendritic Cells - metabolism / Experiments / Health aspects / Homeostasis / Host-Pathogen Interactions / Immunocompromised Host / Inflammatory bowel disease / Interleukin-10 - immunology / Interleukin-10 - metabolism / Interleukins / Lactobacillus casei - immunology / Ligands / Lymphocytes / Medicine / Mice / Mice, Inbred BALB C / Mice, Inbred C57BL / Mice, Knockout / Physiological aspects / Probiotics / Probiotics - administration & dosage / T cells / T-Lymphocytes, Regulatory - cytology / T-Lymphocytes, Regulatory - immunology / T-Lymphocytes, Regulatory - metabolism / Virulence (Microbiology)