Journal Article

On-demand erythrocyte disposal and iron recycling requires transient macrophages in the liver

McKee, Mary, Asshoff, Malte, Theurl, Igor, Swirski, Filip K, Holderried, Tobias A W, Wieghofer, Peter, Prinz, Marco, Wang, Fudi, Rezoagli, Emanuele, Weiss, Guenter, Nahrendorf, Matthias, Babitt, Jodie L, Fenn, Ashley M, Lutz, Oliver M D, Harder, Nina K, Chousterman, Benjamin G, Arvedson, Tara L, McAlpine, Cameron, Theurl, Milan, Hilgendorf, Ingo, Weissleder, Ralph, Nairz, Manfred, Gerhardt, Louisa M S, Haschka, David, Lin, Herbert Y, Rattik, Sara, Seifert, Markus, Iwamoto, Yoshiko, Berra, Lorenzo, Tymoszuk, Piotr, He, Shun, Weber, Georg F, Sopper, Sieghart, Anzai, Atsushi

2016

Overview

Damaged erythrocytes accumulate in various pathological conditions, such as hemolytic anemia, anemia of inflammation, and sickle cell disease. In mice challenged with damaged erythorcytes, a monocyte subset migrates to the liver (but not to the spleen), and this subset differentiates into a transient macrophage population that removes the damaged erythrocytes, thus preventing organ damage. Iron is an essential component of the erythrocyte protein hemoglobin and is crucial to oxygen transport in vertebrates. In the steady state, erythrocyte production is in equilibrium with erythrocyte removal 1 . In various pathophysiological conditions, however, erythrocyte life span is compromised severely, which threatens the organism with anemia and iron toxicity 2 , 3 . Here we identify an on-demand mechanism that clears erythrocytes and recycles iron. We show that monocytes that express high levels of lymphocyte antigen 6 complex, locus C1 (LY6C1, also known as Ly-6C) ingest stressed and senescent erythrocytes, accumulate in the liver via coordinated chemotactic cues, and differentiate into ferroportin 1 (FPN1, encoded by SLC40A1 )-expressing macrophages that can deliver iron to hepatocytes. Monocyte-derived FPN1 + Tim-4 neg macrophages are transient, reside alongside embryonically derived T cell immunoglobulin and mucin domain containing 4 (Timd4, also known as Tim-4) high Kupffer cells (KCs), and depend on the growth factor Csf1 and the transcription factor Nrf2 (encoded by Nfe2l2 ). The spleen, likewise, recruits iron-loaded Ly-6C high monocytes, but these do not differentiate into iron-recycling macrophages, owing to the suppressive action of Csf2. The accumulation of a transient macrophage population in the liver also occurs in mouse models of hemolytic anemia, anemia of inflammation, and sickle cell disease. Inhibition of monocyte recruitment to the liver during stressed erythrocyte delivery leads to kidney and liver damage. These observations identify the liver as the primary organ that supports rapid erythrocyte removal and iron recycling, and uncover a mechanism by which the body adapts to fluctuations in erythrocyte integrity.

• Publisher: Nature Publishing Group US,Nature Publishing Group
• Subject: 13/1 / 13/106 / 13/31 / 631/250/2504/342/1591 / 631/443/319/1557 / 64/60 / Anemia / Anemia, Hemolytic / Anemia, Sickle Cell / Animals / Antigens, Ly - metabolism / Biological research / Biological transport / Biology, Experimental / Biomedicine / Cancer Research / Cation Transport Proteins - metabolism / Cell Differentiation / Disease Models, Animal / Erythrocytes / Erythrocytes - cytology / Erythrocytes - metabolism / Ferroportin / Fluctuations / Granulocyte-Macrophage Colony-Stimulating Factor - metabolism / Hepatocytes - metabolism / Infectious Diseases / Inflammation / Iron / Iron - metabolism / Kidneys / Kupffer Cells - cytology / Kupffer Cells - metabolism / letter / Life span / Liver / Liver - metabolism / Lymphocytes / Macrophage Colony-Stimulating Factor - metabolism / Macrophages - cytology / Macrophages - metabolism / Membrane Proteins - metabolism / Metabolic Diseases / Mice / Molecular Medicine / Monocytes - cytology / Monocytes - metabolism / Neurosciences / NF-E2-Related Factor 2 - metabolism / Nutrient interactions / Oxygen / Physiological aspects / Proteins / Spleen / Vertebrates