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Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies

by Peyrefitte, Christophe , Pastorino, Boris , Dubot-Peres, Audrey , Villarroel, Paola Mariela Saba , Gake, Bouba , de Lamballerie, Xavier , Grard, Gilda , Nurtop, Elif , Gallian, Pierre , Roca, Yelin , Drexler, Jan Felix , Ninove, Laetitia , Priet, Stéphane

in Analysis / Antibodies, Neutralizing / Antibodies, Neutralizing - blood / Antibodies, Viral / Antibodies, Viral - blood / Biomedical and Life Sciences / Biomedicine / blood / Blood & organ donations / Blood donors / cost effectiveness / Cytopathogenic Effect, Viral / dengue / Dengue fever / Diagnosis / Emerging viruses / Encephalitis / Enzyme-Linked Immunosorbent Assay / Enzyme-Linked Immunosorbent Assay - methods / Guillain-Barre syndrome / Humans / Immunoglobulin G / Immunoglobulin G - blood / Infections / Laboratories / Life Sciences / light microscopes / Martinique / Martinique - epidemiology / Mass Screening / Mass Screening - methods / Microbiology and Parasitology / Microcephaly / Microscopy / Neutralization Tests / Neutralization Tests - methods / NS1 protein / Prevalence studies (Epidemiology) / Proteins / Santé publique et épidémiologie / Sensitivity and Specificity / Seroepidemiologic Studies / Seroepidemiology / Serologic Tests / Serologic Tests - methods / seroprevalence / Short Report / Studies / Virology / Virus neutralization test / Zika Virus / Zika Virus - immunology / Zika Virus Infection / Zika Virus Infection - diagnosis / Zika Virus Infection - epidemiology

2018

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Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies

2018

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Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies

2018

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Journal Article

Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies

Peyrefitte, Christophe,

Pastorino, Boris,

Dubot-Peres, Audrey,

Villarroel, Paola Mariela Saba,

Gake, Bouba,

de Lamballerie, Xavier,

Grard, Gilda,

Nurtop, Elif,

Gallian, Pierre,

Roca, Yelin,

Drexler, Jan Felix,

Ninove, Laetitia,

Priet, Stéphane

2018

Overview

Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with a dengue seroprevalence above 90%), this strategy allowed reaching specificity and sensitivity values over 98%. The CPE-based VNT consists of recording CPE directly under the optical microscope, which is easy to identify with ZIKV strain H/PF/2013 at day 5 pi. Overall, considered that CPE-based VNT is cost effective and widely automatable, the NS1 protein-based Zika IgG ELISA+CPE-based VNT combination strategy represents a convenient tool to expedite ZIKV seroprevalence studies.

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Publisher

BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC

Subject

Analysis

/ Antibodies, Neutralizing

/ Antibodies, Neutralizing - blood

/ Antibodies, Viral

/ Antibodies, Viral - blood

/ Biomedical and Life Sciences

/ Biomedicine