Journal Article

Comparison between transcriptomic responses to short-term stress exposures of a common Holarctic and endemic Lake Baikal amphipods

Drozdova, Polina, Schreiber, Stephan, Luckenbach, Till, Axenov-Gribanov, Denis, Jühling, Frank, Hoffmann, Steve, Bedulina, Daria, Vereshchagina, Kseniya, Hackermüller, Jörg, Jakob, Lena, Pörtner, Hans-Otto, Rivarola-Duarte, Lorena, Lucassen, Magnus, Shatilina, Zhanna, Lubyaga, Yulia, Timofeyev, Maxim, Busch, Wibke, Gurkov, Anton, Stadler, Peter F., Otto, Christian, Madyarova, Ekaterina, Sartoris, Franz Josef

2019

Overview

Background Lake Baikal is one of the oldest freshwater lakes and has constituted a stable environment for millions of years, in stark contrast to small, transient bodies of water in its immediate vicinity. A highly diverse endemic endemic amphipod fauna is found in one, but not the other habitat. We ask here whether differences in stress response can explain the immiscibility barrier between Lake Baikal and non-Baikal faunas. To this end, we conducted exposure experiments to increased temperature and the toxic heavy metal cadmium as stressors. Results Here we obtained high-quality de novo transcriptome assemblies, covering mutiple conditions, of three amphipod species, and compared their transcriptomic stress responses. Two of these species, Eulimnogammarus verrucosus and E. cyaneus , are endemic to Lake Baikal, while the Holarctic Gammarus lacustris is a potential invader. Conclusions Both Baikal species possess intact stress response systems and respond to elevated temperature with relatively similar changes in their expression profiles. G. lacustris reacts less strongly to the same stressors, possibly because its transcriptome is already perturbed by acclimation conditions.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Acclimation / Acclimatization / Amhipoda / Amphipoda / Amphipoda - drug effects / Amphipoda - genetics / Amphipoda - physiology / Analysis / Animal biology / Animal Genetics and Genomics / Animals / Baikal / Biochemistry, Molecular Biology / Bioclimatology / Biomedical and Life Sciences / Cadmium / Cadmium - toxicity / Ecology, environment / Ecotoxicology / Environmental aspects / Freshwater lakes / Gene expression / Genetic aspects / Genetic research / Genomics / Geography / Heat shock / Heat-Shock Response - genetics / Heavy metals / High temperature / Immiscibility / Invertebrate Zoology / Lakes / Life Sciences / Microarrays / Microbial Genetics and Genomics / Miscibility / Multicellular invertebrate genomics / Physiological aspects / Plant Genetics and Genomics / Proteomics / Research Article / Species / Species Specificity / Stress (Physiology) / Stress response / Stress, Physiological - drug effects / Stress, Physiological - genetics / Toxicology / Transcriptome - drug effects