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Krüppel-homolog 1 exerts anti-metamorphic and vitellogenic functions in insects via phosphorylation-mediated recruitment of specific cofactors
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Krüppel-homolog 1 exerts anti-metamorphic and vitellogenic functions in insects via phosphorylation-mediated recruitment of specific cofactors
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Journal Article
Krüppel-homolog 1 exerts anti-metamorphic and vitellogenic functions in insects via phosphorylation-mediated recruitment of specific cofactors
2021
Overview
Background
The zinc-finger transcription factor Krüppel-homolog 1 (Kr-h1) exerts a dual regulatory role during insect development by preventing precocious larval/nymphal metamorphosis and in stimulating aspects of adult reproduction such as vitellogenesis. However, how Kr-h1 functions both as a transcriptional repressor in juvenile metamorphosis and an activator in adult reproduction remains elusive. Here, we use the insect
Locusta migratoria
to dissect the molecular mechanism by which Kr-h1 functions as activator and repressor at these distinct developmental stages.
Results
We report that the kinase PKCα triggers Kr-h1 phosphorylation at the amino acid residue Ser
154
, a step essential for its dual functions. During juvenile stage, phosphorylated Kr-h1 recruits a corepressor, C-terminal binding protein (CtBP). The complex of phosphorylated Kr-h1 and CtBP represses the transcription of
Ecdysone induced protein 93F
(
E93
) and consequently prevents the juvenile-to-adult transition. In adult insects, phosphorylated Kr-h1 recruits a coactivator, CREB-binding protein (CBP), and promotes vitellogenesis by inducing the expression of
Ribosomal protein L36
. Furthermore, Kr-h1 phosphorylation with the concomitant inhibition of
E93
transcription is evolutionarily conserved across insect orders.
Conclusion
Our results suggest that Kr-h1 phosphorylation is indispensable for the recruitment of transcriptional cofactors, and for its anti-metamorphic and vitellogenic actions in insects. Our data shed new light on the understanding of Kr-h1 regulation and function in JH-regulated insect metamorphosis and reproduction.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Animals
/ Biomedical and Life Sciences
/ Cyclic AMP response element-binding protein
/ E93
/ Ecdysone
/ Females
/ Gene Expression Regulation, Developmental
/ Homology
/ Insect Proteins - metabolism
/ Insecta
/ Insects
/ Kinases
/ Kr-h1
/ Kruppel-Like Transcription Factors - genetics
/ Kruppel-Like Transcription Factors - metabolism
/ Peptides
/ Proteins
/ Transcription Factors - genetics
