Journal Article

Presence of multiple genotypes in subjects with HPV-16 infection is highly associated with anal squamous intraepithelial lesions in HIV-1 infected males

Galli, Laura, Lazzarin, Adriano, Cernuschi, Massimo, Tambussi, Giuseppe, Castagna, Antonella, Tamburini, Andrea Marco, Albarello, Luca, Rolla, Serena, Rovelli, Cristina, Nozza, Silvia, Poli, Andrea, Rosati, Riccardo, Racca, Sara

2017

Overview

The aim of the study was to determine the prevalence of abnormal cytological findings, high risk (HR)-HPV genotypes and to identify factors associated with an abnormal cytological findings in a cohort of HIV-infected males. Retrospective observational study on HIV-infected male patients who performed screening in the absence of clinical symptoms. Cytological abnormalities were classified as atypical squamous cells of undetermined significance (ASC-US), low-grade(LSIL) or high high-grade squamous intraepithelial lesion (HSIL). Logistic regression models were used to identify predictors of having LSIL/HSIL. Among 875 pts, abnormal cytology findings were observed in 254 (29%, 95% CI: 26.1%-32.1%) subjects: 142 (16%) had LSIL and 49 (6%) HSIL. Overall, 581 (66%, 95%CI: 63.2%-69.5%) subjects had ≥1 HR-HPV type and 269 (31%) had ≥2 HR HPV types. Multivariate logistic regression showed that subjects with multiple HR-HPV genotypes (OR = 1.351, 95%CI: 1.005-2.111) and with HPV-16 type (OR = 2.032, 95%CI: 1.313-3.146) were more likely to have LSIL/HSIL in addition to a lower CD4+/CD8+ ratio, a previous diagnosis of syphilis and a positive viral load. In another multivariate model, the presence of multiple HPV types in subjects with HPV-16 type was associated with the highest adjusted OR of having a LSIL/HSIL (OR = 2.598, 95%CI: 1.460-4.624). In HIV-infected men, the prevalence of abnormal cytological findings was of 29% and of HR-HPV was 66%. The concomitant presence of HPV-16 and multiple HR genotypes was associated with an increased risk of abnormal cytological findings. These data highlight the importance of screening multiple HPV genotypes in HIV-infected patients.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Abnormalities / Acquired immune deficiency syndrome / Adult / AIDS / Alphapapillomavirus - genetics / Anus Neoplasms - complications / Anus Neoplasms - pathology / Anus Neoplasms - virology / Biology and life sciences / Biopsy / Cancer / Care and treatment / CD4 antigen / CD8 antigen / Cellular biology / Cytology / Diagnosis / Gastrointestinal surgery / Gays & lesbians / Genotype / Genotypes / Health screening / HIV / HIV infections / HIV Infections - complications / HIV-1 / Human immunodeficiency virus / Human papillomavirus / Humans / Infections / Infectious diseases / Laboratories / Lesions / Logistic regression / Male / Males / Medical diagnosis / Medical screening / Medicine and Health Sciences / Mens health / Middle Aged / Observational studies / Papillomavirus Infections - complications / Papillomavirus Infections - pathology / Papillomavirus Infections - virology / Patients / People and Places / Precancerous Conditions - complications / Precancerous Conditions - pathology / Precancerous Conditions - virology / Quality / Regression analysis / Regression models / Sexually transmitted diseases / Squamous cells / STD / Studies / Syphilis