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Cruciform DNA in mouse growing oocytes: Its dynamics and its relationship with DNA transcription
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Cruciform DNA in mouse growing oocytes: Its dynamics and its relationship with DNA transcription
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Journal Article
Cruciform DNA in mouse growing oocytes: Its dynamics and its relationship with DNA transcription
2020
Overview
Cruciform DNA is a causing factor of genome instability and chromosomal translocation, however, most studies about cruciform DNA in mammalian cells were based on palindromic sequences containing plasmids and reports about endogenous cruciform DNA are rare. In this study we observed the dynamics of endogenous cruciform DNA in mouse growing oocytes using immunofluorescence labeling method. We found cruciform DNA foci exist in transcription active growing oocytes but not in transcription inactive fully grown oocytes and colocalized with Parp1 but not with DNA damage marker γH2A.X. By analyzing the Genotype-Tissue Expression data, we found cruciform DNA-mediated chromosomal translocation in human spermatocytes is associated with the specific DNA transcription in testis. When inhibiting the transcription with α-amanitin in mouse oocytes, we found oocyte cruciform DNA foci decreased significantly. In summary, we observed the endogenous cruciform DNA in growing oocytes and our results showed that the cruciform DNA formation is transcription-dependent.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
Alpha-Amanitin - adverse effects
/ Amanitin
/ Analysis
/ Animals
/ Antigens
/ DNA
/ Fluorescent Antibody Technique - methods
/ Genomes
/ Genomics
/ Growth
/ Labeling
/ Male
/ Medicine
/ Mice
/ Oocytes
/ Oocytes - growth & development
/ Ovaries
/ Plasmids
/ Poly (ADP-Ribose) Polymerase-1 - metabolism
