Journal Article

Genome-Wide Identification of Genes Essential for the Survival of Streptococcus pneumoniae in Human Saliva

Spagnuolo, Lorenza, Zomer, Aldert, Verhagen, Lilly M., Schraa, Kiki, van der Gaast-de Jongh, Christa E., de Jonge, Marien I., Mennens, Svenja, Eleveld, Marc J., Burghout, Peter, Hermans, Peter W. M., Bootsma, Hester J.

2014

Overview

Since Streptococcus pneumoniae transmits through droplet spread, this respiratory tract pathogen may be able to survive in saliva. Here, we show that saliva supports survival of clinically relevant S. pneumoniae strains for more than 24 h in a capsule-independent manner. Moreover, saliva induced growth of S. pneumoniae in growth-permissive conditions, suggesting that S. pneumoniae is well adapted for uptake of nutrients from this bodily fluid. By using Tn-seq, a method for genome-wide negative selection screening, we identified 147 genes potentially required for growth and survival of S. pneumoniae in saliva, among which genes predicted to be involved in cell envelope biosynthesis, cell transport, amino acid metabolism, and stress response predominated. The Tn-seq findings were validated by testing a panel of directed gene deletion mutants for their ability to survive in saliva under two testing conditions: at room temperature without CO2, representing transmission, and at 37 °C with CO2, representing in-host carriage. These validation experiments confirmed that the plsX gene and the amiACDEF and aroDEBC operons, involved in respectively fatty acid metabolism, oligopeptide transport, and biosynthesis of aromatic amino acids play an important role in the growth and survival of S. pneumoniae in saliva at 37 °C. In conclusion, this study shows that S. pneumoniae is well-adapted for growth and survival in human saliva and provides a genome-wide list of genes potentially involved in adaptation. This notion supports earlier evidence that S. pneumoniae can use human saliva as a vector for transmission.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Amino acids / Bacterial Proteins - genetics / Biology / Biosynthesis / Body fluids / Carbon dioxide / Chromosomes / Clonal deletion / Deletion mutant / Disease transmission / Ear diseases / Fatty acids / Gene deletion / Gene Expression Regulation, Bacterial / Genes / Genetic research / Genome, Bacterial - genetics / Genomes / Genomics / Humans / Infectious diseases / Laboratories / Medicine / Metabolism / Mutants / Negative selection / Nutrient uptake / Nutrients / Operons / Pediatrics / Pneumococcal Infections - genetics / Pneumococcal Infections - microbiology / Pneumococcal Infections - mortality / Pneumonia / Prognosis / Proteins / Respiratory tract / Saliva / Saliva - microbiology / Streptococcus / Streptococcus infections / Streptococcus pneumoniae / Streptococcus pneumoniae - genetics / Streptococcus pneumoniae - growth & development / Streptococcus pneumoniae - pathogenicity / Survival / Survival Rate / Transport / Vaccines