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Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens

by Wang, Yi , Tian, Fenghua , Li, Changtian , Yuan, Xiaolong , Geng, Changan , Hua, Mei

in Acids / Acyl carrier protein / Amino Acid Sequence - genetics / Aspergillus nidulans / Aspergillus oryzae / Base Sequence / Benzofurans - metabolism / Biology / Biology and Life Sciences / Biosynthesis / Chemistry / Claisen cyclase / Computer and Information Sciences / Education / Engineering research / Forestry / Fungi / Gene expression / Genes / Genetic aspects / Genomes / Genomics / Laboratories / Lichens / Lichens - enzymology / Lichens - genetics / Metabolites / Methyltransferase / Nephromopsis / Parmeliaceae - enzymology / Parmeliaceae - genetics / Phylogeny / Physical Sciences / Physiological aspects / Polyketide synthase / Polyketide Synthases - genetics / Polyketides / Proteins / Sequence Analysis, DNA / Usnic acid

2018

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Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens

by Wang, Yi , Tian, Fenghua , Li, Changtian , Yuan, Xiaolong , Geng, Changan , Hua, Mei

2018

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Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens

by Wang, Yi , Tian, Fenghua , Li, Changtian , Yuan, Xiaolong , Geng, Changan , Hua, Mei

2018

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Journal Article

Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens

Wang, Yi,

Tian, Fenghua,

Li, Changtian,

Yuan, Xiaolong,

Geng, Changan,

Hua, Mei

2018

Overview

Usnic acid is a unique polyketide produced by lichens. To characterize usnic acid biosynthesis, the transcriptome of the usnic-acid-producing lichen-forming fungus Nephromopsis pallescens was sequenced using Illumina NextSeq technology. Seven complete non-reducing polyketide synthase genes and nine highly-reducing polyketide synthase genes were obtained through transcriptome analysis. Gene expression results obtained by qPCR and usnic acid detection with LCMS-IT-TOF showed that Nppks7 is probably involved in usnic acid biosynthesis in N. pallescens. Nppks7 is a non-reducing polyketide synthase with a MeT domain that also possesses beta-ketoacyl-ACP synthase, acyl transferase, product template, acyl carrier protein, C-methyltransferase, and Claisen cyclase domains. Phylogenetic analysis shows that Nppks7and other polyketide synthases from lichens form a unique monophyletic clade. Taken together, our data indicate that Nppks7 is a novel PKS in N. pallescens that is likely involved in usnic acid biosynthesis.

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Publisher

Public Library of Science,Public Library of Science (PLoS)

Subject

Acids

/ Acyl carrier protein

/ Amino Acid Sequence - genetics

/ Aspergillus nidulans

/ Aspergillus oryzae

/ Base Sequence

/ Benzofurans - metabolism