Journal Article

Insertion Sequence–Driven Diversification Creates a Globally Dispersed Emerging Multiresistant Subspecies of E. faecium

Leavis, Helen L, van Wamel, Willem J. B, Willems, Rob J. L, Bonten, Marc J. M, Caspers, Martien P. M, Schuren, Frank H

2007

Overview

Enterococcus faecium, an ubiquous colonizer of humans and animals, has evolved in the last 15 years from an avirulent commensal to the third most frequently isolated nosocomial pathogen among intensive care unit patients in the United States. E. faecium combines multidrug resistance with the potential of horizontal resistance gene transfer to even more pathogenic bacteria. Little is known about the evolution and virulence of E. faecium, and genomic studies are hampered by the absence of a completely annotated genome sequence. To further unravel its evolution, we used a mixed whole-genome microarray and hybridized 97 E. faecium isolates from different backgrounds (hospital outbreaks (n = 18), documented infections (n = 34) and asymptomatic carriage of hospitalized patients (n = 15), and healthy persons (n = 15) and animals (n = 21)). Supported by Bayesian posterior probabilities (PP = 1.0), a specific clade containing all outbreak-associated strains and 63% of clinical isolates was identified. Sequencing of 146 of 437 clade-specific inserts revealed mobile elements (n = 74), including insertion sequence (IS) elements (n = 42), phage genes (n = 6) and plasmid sequences (n = 26), hypothetical (n = 58) and membrane proteins (n = 10), and antibiotic resistance (n = 9) and regulatory genes (n = 11), mainly located on two contigs of the unfinished E. faecium DO genome. Split decomposition analysis, varying guanine cytosine content, and aberrant codon adaptation indices all supported acquisition of these genes through horizontal gene transfer with IS16 as the predicted most prominent insert (98% sensitive, 100% specific). These findings suggest that acquisition of IS elements has facilitated niche adaptation of a distinct E. faecium subpopulation by increasing its genome plasticity. Increased genome plasticity was supported by higher diversity indices (ratio of average genetic similarities of pulsed-field gel electrophoresis and multi locus sequence typing) for clade-specific isolates. Interestingly, the previously described multi locus sequence typing-based clonal complex 17 largely overlapped with this clade. The present data imply that the global emergence of E. faecium, as observed since 1990, represents the evolution of a subspecies with a presumably better adaptation than other E. faecium isolates to the constraints of a hospital environment.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Antibiotic resistance / Antimicrobial agents / Base Sequence / Confidence intervals / Diversification / Diversity indices / DNA Transposable Elements / Drug resistance / Drug Resistance, Multiple, Bacterial / Enterococcus / Enterococcus faecium / Enterococcus faecium - classification / Enterococcus faecium - drug effects / Enterococcus faecium - genetics / Eubacteria / Evolution, Molecular / Evolutionary Biology / Gene Rearrangement / Genes / Genome, Bacterial / Genomes / Infections / Intensive care / Medical research / Microbiology / Molecular Biology / Molecular Sequence Data / Mosaicism / Nosocomial infection / Oligonucleotide Array Sequence Analysis / Open Reading Frames / Outbreaks / Phylogenetics / Phylogeny / Plasticity / R&D / Recombination, Genetic / Research & development / Varieties