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Development of a promising antigenic cocktail for the global detection of Babesia caballi in horse by ELISA
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Development of a promising antigenic cocktail for the global detection of Babesia caballi in horse by ELISA
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Development of a promising antigenic cocktail for the global detection of Babesia caballi in horse by ELISA
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Journal Article
Development of a promising antigenic cocktail for the global detection of Babesia caballi in horse by ELISA
2023
Overview
In this study, we designed novel truncated Babesia caballi ( B . caballi ) recombinant proteins from the previously used B . caballi proteins; 134-Kilodalton Protein (rBC134) and Merozoite Rhoptry 48 Protein (rBC48). Then, we evaluated the diagnostic performance of the newly designed proteins when used as a single antigen or when used as cocktail antigen consists of rBC134 full length (rBC134f) + newly designed rBC48 (rBC48t) or newly designed rBC134 (rBC134t) + rBC48t for the detection of B . caballi infection in horse using indirect enzyme-linked immunosorbent assay (iELISA). We used one dose and a half of each antigen in the cocktail formulas. The serum samples were collected from different endemic areas in addition to the sera collected from horses experimentally infected with B . caballi were used in the present study. Cocktail antigen in full dose of (rBC134f + rBC48t) exhibited the highest optical density (OD) values with B . caballi –infected sera and showed the lowest OD values with normal equine sera or B . caballi , and Theileria equi mixed infected sera in comparison with the single antigen. Interestingly, the same cocktail antigen exhibited the highest concordance rate (76.74%) and kappa value (0.79) in the screening of 200 field serum samples collected from five B . caballi endemic countries, including South Africa (n = 40), Ghana (n = 40), Mongolia (n = 40), Thailand (n = 40), and China (n = 40) using iELISA and the results were compared to those of indirect fluorescent antibody test (IFAT) as a reference. Moreover, the identified promising cocktail full dose antigen (rBC134f + rBC48t) showed that it can detect the infection as early as the 4 th day post-infection in sera collected from experimentally infected horses. The obtained results revealed the reliability of the rBC134f + rBC48t cocktail antigen when used in full dose for the detection of specific antibodies to B . caballi in horses which will be useful for epidemiological surveys and control of equine babesiosis .
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Analysis
/ Animals
/ Antigens
/ Babesia
/ Cattle
/ E coli
/ Enzyme-linked immunosorbent assay
/ Enzyme-Linked Immunosorbent Assay - veterinary
/ Enzymes
/ Horse Diseases - epidemiology
/ Horses
/ Indirect fluorescent antibody test
/ Medicine and Health Sciences
/ Proteins
/ Research and Analysis Methods
/ Serology
/ Values
