Journal Article

The Initial Inflammatory Response to Bioactive Implants Is Characterized by NETosis

Klappacher, Michaela, Vitkov, Ljubomir, Krautgartner, Wolf-Dietrich, Obermayer, Astrid, Stoiber, Walter, Hartl, Dominik, Hannig, Matthias

2015

Overview

Implants trigger an inflammatory response, which is important for osseointegration. Here we studied neutrophil extracellular trap (NET) release of human neutrophils in response to sandblasted large-grit acid etched (SLA) implants using fluorescent, confocal laser scanning and scanning electron microscopy. Our studies demonstrate that human neutrophils rapidly adhered to SLA surfaces, which triggered histone citrullination and NET release. Further studies showed that albumin or acetylsalicylic acid had no significant effects on the inflammatory response to SLA surfaces. In contrast to bioinert materials, which do not osseointegrate, the bioactivity of SLA surfaces is coupled with the ability to release NETs. Further investigations are necessary for clarifying the role of NETosis for osseointegration.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Acetylsalicylic acid / Acids / Adult / Aspirin / Biological activity / Biology / Blood & organ donations / Blood platelets / Citrulline / Confocal / Dentistry / Electron microscopy / Extracellular Traps - drug effects / Female / Fluorescence / Grit / Histones - metabolism / Human behavior / Humans / Implants / Implants, Experimental - adverse effects / Inflammation / Inflammation - etiology / Inflammatory response / Joint surgery / Leukocytes (neutrophilic) / Male / Nanoparticles / Neutrophils / Neutrophils - drug effects / Neutrophils - metabolism / Neutrophils - ultrastructure / Osseointegration / Scanning electron microscopy / Studies / Titanium - pharmacology / Transplants & implants / Zoology