Journal Article

Recruitment of Vps34 PI3K and enrichment of PI3P phosphoinositide in the viral replication compartment is crucial for replication of a positive-strand RNA virus

Kovalev, Nikolay, Feng, Zhike, Xu, Kai, Nagy, Peter D.

2019

Overview

Tombusviruses depend on subversions of multiple host factors and retarget cellular pathways to support viral replication. In this work, we demonstrate that tomato bushy stunt virus (TBSV) and the closely-related carnation Italian ringspot virus (CIRV) recruit the cellular Vps34 phosphatidylinositol 3-kinase (PI3K) into the large viral replication compartment. The kinase function of Vps34 is critical for TBSV replication, suggesting that PI(3)P phosphoinositide is utilized by TBSV for building of the replication compartment. We also observed increased expression of Vps34 and the higher abundance of PI(3)P in the presence of the tombusviral replication proteins, which likely leads to more efficient tombusvirus replication. Accordingly, overexpression of PI(3)P phosphatase in yeast or plants inhibited TBSV replication on the peroxisomal membranes and CIRV replication on the mitochondrial membranes. Moreover, the purified PI(3)P phosphatase reduced TBSV replicase assembly in a cell-free system. Detection of PI(3)P with antibody or a bioprobe revealed the enrichment of PI(3)P in the replication compartment. Vps34 is directly recruited into the replication compartment through interaction with p33 replication protein. Gene deletion analysis in surrogate yeast host unraveled that TBSV replication requires the vesicle transport function of Vps34. In the absence of Vps34, TBSV cannot efficiently recruit the Rab5-positive early endosomes, which provide PE-rich membranes for membrane biogenesis of the TBSV replication compartment. We found that Vps34 and PI(3)P needed for the stability of the p33 replication protein, which is degraded by the 26S proteasome when PI(3)P abundance was decreased by an inhibitor of Vps34. In summary, Vps34 and PI(3)P are needed for providing the optimal microenvironment for the replication of the peroxisomal TBSV and the mitochondrial CIRV.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: 1-Phosphatidylinositol 3-kinase / Antibodies / Autophagy / Binding sites / Biology and Life Sciences / Biosynthesis / Bushy stunt / Cell-free system / Class III Phosphatidylinositol 3-Kinases - genetics / Class III Phosphatidylinositol 3-Kinases - metabolism / Clonal deletion / Development and progression / Endosomes / Enrichment / Gene deletion / Genetic aspects / Genetic research / Hepatitis / Kinases / Lipids / Medicine and Health Sciences / Membranes / Mitochondria / Mitochondria - metabolism / Mitochondrial Membranes - metabolism / Parasites / Peroxisomes - metabolism / Phosphatases / Phosphatidylinositol phosphates / Phosphatidylinositol Phosphates - genetics / Phosphatidylinositol Phosphates - metabolism / Phosphatidylinositols / Phospholipids / Physiological aspects / Plant pathology / Proteasome 26S / Proteins / Recruitment / Replicase / Replication / Research and Analysis Methods / RNA / RNA Replicase - metabolism / RNA virus infections / RNA viruses / RNA Viruses - genetics / RNA, Viral - genetics / Tomatoes / Tombusvirus - genetics / Tombusvirus - metabolism / Trends / Tuberculosis / Viral Proteins - metabolism / Virology / Virus replication / Virus Replication - genetics / Viruses / Yeast