Journal Article

Towards a molecular basis of ubiquitin signaling: A dual-scale simulation study of ubiquitin dimers

Peter, Christine, Kukharenko, Oleksandra, Berg, Andrej, Scheffner, Martin

2018

Overview

Covalent modification of proteins by ubiquitin or ubiquitin chains is one of the most prevalent post-translational modifications in eukaryotes. Different types of ubiquitin chains are assumed to selectively signal respectively modified proteins for different fates. In support of this hypothesis, structural studies have shown that the eight possible ubiquitin dimers adopt different conformations. However, at least in some cases, these structures cannot sufficiently explain the molecular basis of the selective signaling mechanisms. This indicates that the available structures represent only a few distinct conformations within the entire conformational space adopted by a ubiquitin dimer. Here, molecular simulations on different levels of resolution can complement the structural information. We have combined exhaustive coarse grained and atomistic simulations of all eight possible ubiquitin dimers with a suitable dimensionality reduction technique and a new method to characterize protein-protein interfaces and the conformational landscape of protein conjugates. We found that ubiquitin dimers exhibit characteristic linkage type-dependent properties in solution, such as interface stability and the character of contacts between the subunits, which can be directly correlated with experimentally observed linkage-specific properties.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Algorithms / Biology and Life Sciences / Cellular signal transduction / Chains / Chemistry / Computer Simulation / Databases, Protein / Dimers / Eukaryotes / Image retrieval / Interface stability / Interfaces / Mathematical models / Methods / Models, Molecular / Molecular Dynamics Simulation / Physical Sciences / Physiological aspects / Post-translation / Protein Binding / Protein Interaction Mapping / Protein Multimerization / Protein Processing, Post-Translational / Proteins / Research and Analysis Methods / Signal Transduction / Signaling / Simulation / Ubiquitin / Ubiquitin - chemistry / Ubiquitination