Asset Details
Do you wish to reserve the book?
Conversion of lignin model compounds by Pseudomonas putida KT2440 and isolates from compost
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Conversion of lignin model compounds by Pseudomonas putida KT2440 and isolates from compost
Do you wish to request the book?
Conversion of lignin model compounds by Pseudomonas putida KT2440 and isolates from compost
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Conversion of lignin model compounds by Pseudomonas putida KT2440 and isolates from compost
2017
Overview
Starting from mature vegetable compost, four bacterial strains were selected using a lignin-rich medium. 16S ribosomal RNA identification of the isolates showed high score similarity with
Pseudomonas
spp. for three out of four isolates. Further characterization of growth on mixtures of six selected lignin model compounds (vanillin, vanillate, 4-hydroxybenzoate,
p
-coumarate, benzoate, and ferulate) was carried out with three of the
Pseudomonas
isolates and in addition with the strain
Pseudomonas putida
KT2440 from a culture collection. The specific growth rates on benzoate,
p
-coumarate, and 4-hydroxybenzoate were considerably higher (0.26–0.27 h
−1
) than those on ferulate and vanillate (0.21 and 0.22 h
−1
), as were the uptake rates. There was no direct growth of
P. putida
KT2440 on vanillin, but instead, vanillin was rapidly converted into vanillate at a rate of 4.87 mmol (g
CDW
h)
−1
after which the accumulated vanillate was taken up. The growth curve reflected a diauxic growth when mixtures of the model compounds were used as carbon source. Vanillin, 4-hydroxybenzoate, and benzoate were preferentially consumed first, whereas ferulate was always the last substrate to be taken in. These results contribute to a better understanding of the aromatic metabolism of
P. putida
in terms of growth and uptake rates, which will be helpful for the utilization of these bacteria as cell factories for upgrading lignin-derived mixtures of aromatic molecules.
Publisher
Springer Berlin Heidelberg,Springer,Springer Nature B.V
Subject
Applied Microbial and Cell Physiology
/ Bacteria
/ Bacterial Proteins - metabolism
/ Benzaldehydes - pharmacology
/ Biologi
/ Biomedical and Life Sciences
/ Carbon
/ Composts
/ Coumaric Acids - pharmacology
/ Lignin
/ Microbial Genetics and Genomics
/ Pseudomonas putida - drug effects
/ Pseudomonas putida - genetics
/ Pseudomonas putida - growth & development
/ Pseudomonas putida - metabolism
/ RNA
/ rRNA 16S
/ Vanillin
