Journal Article

Quantitative monitoring of paramagnetic contrast agents and their allocation in plant tissues via DCE-MRI

Hammer, Sebastian, Guendel, Andre, Mayer, Simon, Jakob, Peter M., Munz, Eberhard, Rolletschek, Hardy, Borisjuk, Ljudmilla, Neuberger, Thomas, Wagner, Steffen

2022

Overview

Background Studying dynamic processes in living organisms with MRI is one of the most promising research areas. The use of paramagnetic compounds as contrast agents (CA), has proven key to such studies, but so far, the lack of appropriate techniques limits the application of CA-technologies in experimental plant biology. The presented proof-of-principle aims to support method and knowledge transfer from medical research to plant science. Results In this study, we designed and tested a new approach for plant Dynamic Contrast Enhanced Magnetic Resonance Imaging (pDCE-MRI). The new approach has been applied in situ to a cereal crop ( Hordeum vulgare ). The pDCE-MRI allows non-invasive investigation of CA allocation within plant tissues. In our experiments, gadolinium-DTPA, the most commonly used contrast agent in medical MRI, was employed. By acquiring dynamic T 1 -maps, a new approach visualizes an alteration of a tissue-specific MRI parameter T 1 (longitudinal relaxation time) in response to the CA. Both, the measurement of local CA concentration and the monitoring of translocation in low velocity ranges (cm/h) was possible using this CA-enhanced method. Conclusions A novel pDCE-MRI method is presented for non-invasive investigation of paramagnetic CA allocation in living plants. The temporal resolution of the T 1 -mapping has been significantly improved to enable the dynamic in vivo analysis of transport processes at low-velocity ranges, which are common in plants. The newly developed procedure allows to identify vascular regions and to estimate their involvement in CA allocation. Therefore, the presented technique opens a perspective for further development of CA-aided MRI experiments in plant biology.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Analysis / Barley / Biological Techniques / Biology / Biomedical and Life Sciences / biomedical research / Cereal crops / Contrast agent (CA) / Contrast agents / Contrast media / DCE-MRI / Experiments / Gadolinium / Gadolinium DTPA / Gene expression / Growth / Hordeum vulgare / Knowledge management / Life Sciences / Magnetic resonance imaging / Magnetic resonance imaging (MRI) / magnetism / Medical research / Methods / Monitoring / Plant biology / Plant Sciences / Plant tissues / Relaxation time / Temporal resolution / Translocation / Transport processes / Vascular bundles / Velocity