Journal Article

Mathematical modeling of biohydrogen production via dark fermentation of fruit peel wastes by Clostridium butyricum NE95

Elerakey, Norhan, Hawary, Heba, Aboseidah, Akram A., Rasmey, Abdel-Hamied M.

2024

Overview

Background Biohydrogen production from agro-industrial wastes through dark fermentation offers several advantages including eco-friendliness, sustainability, and the simplicity of the process. This study aimed to produce biohydrogen from fruit and vegetable peel wastes (FVPWs) by anaerobic fermentative bacteria isolated from domestic wastewater. Kinetic analysis of the produced biohydrogen by five isolates on a glucose medium was analyzed using a modified Gompertz model (MGM). Besides, the feasibility of hydrogen production by Clostridium butyricum NE95 using FVPWs as substrates was investigated. Results The bacterial isolate NE95 was selected as the highest biohydrogen producer with maximum biohydrogen production (H max ) of 1617.67 ± 3.84 mL/L, R max (MGM) of 870.77 mL/L/h and lag phase (λ) of 28.37 h. NE95 was phenotypically and genetically identified as C. butyricum and its 16 S rRNA gene sequence was deposited in the GenBank under the accession number PP581833. The genetic screening of hydrogenase gene clusters indicated the presence of Fe-Fe hydrogenase gene in C. butyricum NE95. C. butyricum NE95 showed the ability to produce biohydrogen from different FVPWs, with watermelon and melon peels being the most promising feedstocks for fermentation. It was revealed that using a mixture (1:1, w/w) of watermelon and melon peels as a substrate for C. butyricum NE95 significantly increased biohydrogen yield with H max of 991.00 ± 10.54 mL/L, R max of 236.31 mL/L/h, λ of 33.92 h and a high accuracy of R 2 (0.997). Conclusions The study highlights the effectiveness of C. butyricum NE95 on the valorization of FVPWs and generates a sustainable source of biohydrogen production.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Agricultural wastes / Anaerobic processes / Applied Microbiology / Bacteria / Biochemical Engineering / Biohydrogen / Biological products / Biomedical Engineering/Biotechnology / Biotechnology / Carbon / Chemical properties / Chemistry / Chemistry and Materials Science / Clostridium / Clostridium butyricum / Clostridium butyricum - genetics / Clostridium butyricum - metabolism / Domestic wastewater / Emissions / feedstocks / Fermentation / Fruit - metabolism / Fruit - microbiology / Fruit and vegetable peel wastes / fruit peels / Fruits / Gene clusters / genes / Genetic Engineering / Genetic screening / Glucose / Gram-positive bacteria / Greenhouse gases / Household wastes / Hydrogen / Hydrogen - metabolism / Hydrogen production / Hydrogenase / Hydrogenase - genetics / Hydrogenase - metabolism / Industrial wastes / Kinetic analysis / kinetics / logit analysis / Mathematical models / Maximum likelihood method / Methods / Microbial cell bioprocessing and biomanufacturing / Models, Theoretical / municipal wastewater / Nitrogen / nucleotide sequences / Plant Breeding/Biotechnology / Production processes / Purification / RNA, Ribosomal, 16S - genetics / rRNA / Sewage / Substrates / Sustainability / vegetable peels / Water melons / watermelons