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The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends
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Journal Article
The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends
2006
Overview
Hang on in there
A long-standing mystery of mitosis research is the mechanism that transports chromosomes to the nuclear-spindle poles during anaphase. The chromosomes seem to gain their objective by clinging on to the kinetochore microtubule polymers despite the fact that they are disassembling at the time. Fluorescence microscopy has now been used to produce movies and electron micrographs that show how it's done. The microtubule polymer disassembles via a conformational change that pushes the Dam1 ring complex, an important microtubule binding element in the budding yeast kinetochore, along its lattice. This elegant mechanism may be the key to the conversion of force generated by microtubule depolymerization into the chromosome movements seen in mitosis.
The Dam1 ring complex is a molecular device that can translate the force generated by microtubule depolymerization into movement along the lattice to facilitate chromosome segregation.
Chromosomes interact through their kinetochores with microtubule plus ends and they are segregated to the spindle poles as the kinetochore microtubules shorten during anaphase A of mitosis. The molecular natures and identities of coupling proteins that allow microtubule depolymerization to pull chromosomes to poles during anaphase have long remained elusive
1
. In budding yeast, the ten-protein Dam1 complex is a critical microtubule-binding component of the kinetochore
2
that oligomerizes into a 50-nm ring around a microtubule
in vitro
3
,
4
. Here we show, with the use of a real-time, two-colour fluorescence microscopy assay, that the ring complex moves processively for several micrometres at the ends of depolymerizing microtubules without detaching from the lattice. Electron microscopic analysis of ‘end-on views’ revealed a 16-fold symmetry of the kinetochore rings. This out-of-register arrangement with respect to the 13-fold microtubule symmetry is consistent with a sliding mechanism based on an electrostatically coupled ring–microtubule interface. The Dam1 ring complex is a molecular device that can translate the force generated by microtubule depolymerization into movement along the lattice to facilitate chromosome segregation.
Publisher
Nature Publishing Group UK,Nature Publishing,Nature Publishing Group
Subject
Biological and medical sciences
/ Cell Cycle Proteins - physiology
/ Cell structures and functions
/ Chromosome Segregation - physiology
/ Cytoskeleton, cytoplasm. Intracellular movements
/ Fundamental and applied biological sciences. Psychology
/ Humanities and Social Sciences
/ Kinetochores - ultrastructure
/ letter
/ Microtubule-Associated Proteins - physiology
/ Microtubules - ultrastructure
/ Molecular and cellular biology
/ Movement
/ Proteins
/ Saccharomyces cerevisiae Proteins - physiology
/ Science
/ Spindle Apparatus - physiology
/ Spindle Apparatus - ultrastructure
/ Yeast
/ Yeasts
