Journal Article

Transcriptome analysis and genome-wide identification of WRKY gene family in Leonurus japonicus under drought stress

Yi, Yuxiao, Guo, Tongtong, Yang, Fengtang, Xu, Lili, Yin, Zelin, Wang, Qing, Xu, Jianing, Zhang, Ming

2025

Overview

Background Leonurus japonicus ( L. japonicus ) is a herbaceous flowering plant, widely distributed in Asia. Drought is one of the primary environmental stress factors affecting L. japonicus growth. Previous studies have demonstrated that WRKY transcription factors (TFs) play a crucial role in plant responses to drought stress. So far, there has been no research on the function of WRKY genes in L. japonicus . Results The physiological experiment results showed that drought stress significantly increased the malondialdehyde (MDA), proline, and hydrogen peroxide (H₂O₂) content of L. japonicus . Transcriptome analysis revealed significant changes in the expression levels of the WRKY gene family. Based on bioinformatics analysis, 67 WRKY genes ( LjWRKYs ) were identified in the genome of L. japonicus , with amino acid lengths ranging from 85 to 574. The LjWRKYs can be divided into three subfamilies. Among them, the expression of LjWRKY (1/4/23/44) were significantly up-regulated under drought stress, whereas the expression of LjWRKY (21/25/65) were significantly down-regulated. Additionally, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that after drought stress, differentially expressed genes (DEGs) were enriched in plant hormone signal transduction pathway, the MAPK signaling pathway and biosynthesis of secondary metabolites pathway. In the MAPK pathway, there were 19 DEGs, 9 of which contained W-box regions, suggesting that they may be potential regulatory targets of LjWRKY TFs under drought stress. Conclusion These findings suggested that WRKY gene family may participate in the response to drought stress in L. japonicus . This study provides a scientific basis for the further development and functional validation of the WRKY gene family in L. japonicus. Clinical trial number Not applicable.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Abiotic stress / Agriculture / Amino acids / Anopheles / Antioxidants / Asia / Bioinformatics / Biomedical and Life Sciences / Biosynthesis / Botanical research / China / clinical trials / Dehydration / DNA binding proteins / Drought / Drought stress / Droughts / Environmental aspects / Environmental stress / family / Flowering / Flowering plants / Flowers & plants / Gene expression / Gene Expression Profiling / gene expression regulation / Gene Expression Regulation, Plant / Genes / Genetic aspects / Genome, Plant / Genome-wide analysis / Genomes / Genomic analysis / Genomics / Hydrogen peroxide / Kinases / Lamiaceae / Leonurus - genetics / Leonurus - metabolism / Leonurus - physiology / Leonurus japonicus / Life Sciences / malondialdehyde / MAP kinase / Metabolism / Metabolites / Multigene Family / Phylogeny / Physiological aspects / Physiology / Plant genetics / Plant hormones / Plant Proteins - genetics / Plant Proteins - metabolism / Plant Sciences / Polyethylene glycol / Proline / Proteins / Secondary metabolites / Signal transduction / Stress, Physiological - genetics / Transcription factors / Transcription Factors - genetics / Transcription Factors - metabolism / Transcriptomes / transcriptomics / Tree Biology / water stress / WRKY family