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G-protein Coupled Receptor 34 Knockdown Impairs the Proliferation and Migration of HGC-27 Gastric Cancer Cells In Vitro
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G-protein Coupled Receptor 34 Knockdown Impairs the Proliferation and Migration of HGC-27 Gastric Cancer Cells In Vitro
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G-protein Coupled Receptor 34 Knockdown Impairs the Proliferation and Migration of HGC-27 Gastric Cancer Cells In Vitro
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Journal Article
G-protein Coupled Receptor 34 Knockdown Impairs the Proliferation and Migration of HGC-27 Gastric Cancer Cells In Vitro
2015
Overview
Background：Overexpression of G-protein coupled receptor 34 （GPR34） affects the progression and prognosis of human gastric adenocarcinoma,however,the role of GPR34 in gastric cancer development and progression has not been well-determined.The current study aimed to investigate the effect of GPR34 knockdown on the proliferation,migration,and apoptosis of HGC-27 gastric cancer cells and the underlying mechanisms.Methods：The expression of GPR34 in gastric cancer cell line HGC-27 was detected by quantitative real-time reverse transcription-polymerase chain reaction （RT-PCR） and Western blotting.HGC-27 cells were employed to construct the stable GPR34 knockdown cell model in this study.Real-time RT-PCR and Western blotting were applied to validate the effect of short hairpin RNA （ShRNA） on the expression of GPR34 in HGC-27 gastric cells.The proliferation,migration of these cells were examined by Cell Counting Kit-8 and transwell.We also measured expression profile of PI3K/PDK1/AKT and ERK using Western blotting.Results：The ShRNA directed against GPR34 effectively inhibited both endogenous mRNA and protein expression levels of GPR34,and significantly down-regulated the expression of PIK3CB （P ＜ 0.01）,PIK3CD （P ＜ 0.01）,PDK1 （P ＜ 0.01）,phosphorylation of PDK1 （P ＜ 0.01）,Akt （P ＜ 0.01）,and ERK （P ＜ 0.01）.Furthermore,GPR34 knockdown resulted in an obvious reduction in HGC-27 cancer cell proliferation and migration activity （P ＜ 0.01）.Conclusions：GPR34 knockdown impairs the proliferation and migration of HGC-27 gastric cancer cells in vitro and provides a potential implication for therapy of gastric cancer.
Publisher
Medknow Publications Pvt Ltd,Medknow Publications and Media Pvt. Ltd,Lippincott Williams & Wilkins Ovid Technologies,Department of Hepatobiliary Surgery, Peking University People's Hospital, Beijing 100044, China%Department of Gastroenterology, Peking University People's Hospital, Beijing 100044, China%Institute of Clinical Molecular Biology, Peking University People's Hospital, Beijing 100044, China%Department of General Surgery, Beijing Changping District Hospital, Beijing 102200, China,Medknow Publications & Media Pvt Ltd,Wolters Kluwer
Subject
/ Cell Proliferation - genetics
/ Cell Proliferation - physiology
/ Gastric Cancer; GPR34; Knockdown; Migration; Proliferation
/ G蛋白偶联受体
/ Humans
/ Kinases
/ Lymphoma
/ Melanoma
/ Original
/ Proteins
/ Real-Time Polymerase Chain Reaction
/ Receptors, Lysophospholipid - genetics
/ Receptors, Lysophospholipid - metabolism
/ RNA, Small Interfering - genetics
/ shRNA
/ Stomach Neoplasms - genetics
/ Stomach Neoplasms - metabolism
/ Studies
/ Surgery
/ 损害
/ 细胞增殖
/ 胃癌细胞
/ 迁移
