Journal Article

Characterization and Screening of Native Scenedesmus sp. Isolates Suitable for Biofuel Feedstock

Gour, Rakesh Singh, Chauhan, Rajinder Singh, Kant, Anil, Singh, Harvinder, Chawla, Aseem

2016

Overview

In current study isolates of two native microalgae species were screened on the basis of growth kinetics and lipid accumulation potential. On the basis of data obtained on growth parameters and lipid accumulation, it is concluded that Scenedesmus dimorphus has better potential as biofuel feedstock. Two of the isolates of Scenedesmus dimorphus performed better than other isolates with respect to important growth parameters with lipid content of ~30% of dry biomass. Scenedesmus dimorphus was found to be more suitable as biodiesel feedstock candidate on the basis of cumulative occurrence of five important biodiesel fatty acids, relative occurrence of SFA (53.04%), MUFA (23.81%) and PUFA (19.69%), and more importantly that of oleic acid in its total lipids. The morphological observations using light and Scanning Electron Microscope and molecular characterization using amplified 18S rRNA gene sequences of microalgae species under study were also performed. Amplified 18S rRNA gene fragments of the microalgae species were sequenced, annotated at the NCBI website and phylogenetic analysis was done. We have published eight 18S rRNA gene sequences of microalgae species in NCBI GenBank.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Accumulation / Algae / Alternative energy sources / Analysis / Aquatic microorganisms / Biodiesel fuels / Biofuels / Bioinformatics / Biology and Life Sciences / Biomass / Biomass energy / Biotechnology / Chlorella / Chlorophyta / Classification / Diesel / DNA - chemistry / DNA Primers / Ecology and Environmental Sciences / Engineering and Technology / Fatty Acids / Fatty Acids, Unsaturated - chemistry / Fuels / Gas Chromatography-Mass Spectrometry / Gene sequencing / Genetic aspects / Growth kinetics / Growth rate / Identification / India / Indigenous species / Information technology / Kinetics / Lipids / Lipids - chemistry / Medical screening / Microalgae / Microalgae - growth & development / Microalgae - metabolism / Microscopy, Electron, Scanning / Oleic acid / Parameters / Photosynthesis / Phylogenetics / Phylogeny / Physical Sciences / Physiological aspects / Polymerase Chain Reaction / Polyunsaturated fatty acids / Production processes / Productivity / Raw materials / Research and Analysis Methods / Ribosomal DNA / RNA, Ribosomal, 18S - chemistry / rRNA 18S / Scanning electron microscopy / Scenedesmus / Scenedesmus - growth & development / Scenedesmus - metabolism / Scenedesmus dimorphus / Scenedesmus quadricauda / Studies / Taxonomy