Journal Article

Gut microbiota composition alterations are associated with the onset of diabetes in kidney transplant recipients

Tourret, Jérôme, Lecronier, Marie, Denamur, Erick, Tamzali, Yanis, Barrou, Benoit, Tashk, Parvine, Tenaillon, Olivier, Aron-Wisnewsky, Judith

2020

Overview

Patients transplanted at our institution provided fecal samples before, and 3-9 months after KT. Fecal bacterial DNA was extracted and 9 bacteria or bacterial groups were quantified by qPCR. 50 patients (19 controls without diabetes, 15 who developed New Onset Diabetes After Transplantation, NODAT, and 16 with type 2 diabetes before KT) were included. Before KT, Lactobacillus sp. tended to be less frequently detected in controls than in those who would become diabetic following KT (NODAT) and in initially diabetic patients (60%, 87.5%, and 100%, respectively, p = 0.08). The relative abundance of Faecalibacterium prausnitzii was 30 times lower in initially diabetic patients than in controls (p = 0.002). The relative abundance of F. prausnitzii of NODAT patients was statistically indistinguishable from controls and from diabetic patients. The relative abundance of Lactobacillus sp. increased following KT in NODAT and in initially diabetic patients (20-fold, p = 0.06, and 25-fold, p = 0.02, respectively). In contrast, the proportion of Akkermansia muciniphila decreased following KT in NODAT and in initially diabetic patients (2,500-fold, p = 0.04, and 50,000-fold, p<0.0001, respectively). The proportion of Lactobacillus and A. muciniphila did not change in controls between before and after the transplantation. Consequently, after KT the relative abundance of Lactobacillus sp. was 25 times higher (p = 0.07) and the relative abundance of A. muciniphila was 2,000 times lower (p = 0.002) in diabetics than in controls. An alteration of the gut microbiota composition involving Lactobacillus sp., A. muciniphila and F. prausnitzii is associated with the glycemic status in KT recipients, raising the question of their role in the genesis of NODAT.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Abundance / Antibiotics / Bacteria / Bacterial genetics / Basiliximab / Biology and Life Sciences / Composition / Deoxyribonucleic acid / Diabetes / Diabetes mellitus / Diabetes mellitus (non-insulin dependent) / Diabetes Mellitus, Type 2 - complications / Diabetes Mellitus, Type 2 - metabolism / Diabetes Mellitus, Type 2 - microbiology / Diabetes Mellitus, Type 2 - pathology / Diabetes therapy / Diabetics / Disease / DNA / DNA, Bacterial - genetics / Dysbacteriosis / Faecalibacterium prausnitzii - genetics / Faecalibacterium prausnitzii - isolation & purification / Faecalibacterium prausnitzii - metabolism / Feces / Feces - microbiology / Female / Gastrointestinal Microbiome - genetics / Gastrointestinal surgery / Genetic testing / Glucose / Gut microbiota / Hemoglobin / Human health and pathology / Humans / Hépatology and Gastroenterology / Insulin resistance / Intestinal microflora / Kidney transplantation / Kidney Transplantation - adverse effects / Kidney transplants / Kidneys / Lactobacillus / Lactobacillus - genetics / Lactobacillus - isolation & purification / Lactobacillus - metabolism / Life Sciences / Male / Medicine and Health Sciences / Metabolic disorders / Microbiota / Microbiota (Symbiotic organisms) / Middle Aged / Nutrition / Obesity / Organ transplant recipients / Organ transplantation / Relative abundance / Risk Factors / Statistical methods / Transplantation / Transplants & implants / Type 2 diabetes / Urology and Nephrology / Verrucomicrobia - genetics / Verrucomicrobia - isolation & purification / Verrucomicrobia - metabolism