Journal Article

Between-cow variation in milk fatty acids associated with methane production

de Souza, J., Leskinen, H., Lock, A. L., Shingfield, K. J., Huhtanen, P.

2020

Overview

We evaluated the between-cow (b-cow) variation and repeatability in omasal and milk fatty acids (FA) related to methane (CH4) emission. The dataset was originated from 9 studies with rumen-cannulated dairy cows conducted using either a switch-back or a Latin square design. Production of CH4 per mole of VFA (Y_CH4VFA) was calculated based on VFA stoichiometry. Experiment, diet within experiment, period within experiment, and cow within experiment were considered as random factors. Empirical models were developed between the variables of interest by univariate and bivariate mixed model regression analysis. The variation associated with diet was higher than the b-cow variation with low repeatability (< 0.25) for milk odd- and branch-chain FA (OBCFA). Similarly, for de novo synthesized milk FA, diet variation was ~ 3-fold greater than the b-cow variation; repeatability for these FA was moderate to high (0.34-0.58). Also, for both cis-9 C18:1 and cis-9 cis-12 cis-15 C18:3 diet variation was more than double the b-cow variation, but repeatability was moderate. Among the de novo milk FA, C4:0 was positively related with stoichiometric Y_CH4VFA, while for OBCFA, anteiso C15:0 and C15:0 were negatively related with it. Notably, when analyzing the relationship between omasal FA and milk FA we observed positive intercept estimates for all the OBCFA, which may indicate endogenous post-ruminal synthesis of these FA, most likely in the mammary gland. For milk iso C13:0, iso C15:0, anteiso C15:0, and C15:0 were positively influenced by omasal proportion of their respective FA and by energy balance. In contrast, the concentration of milk C17:0, iso C18:0, C18:0, cis-11 C18:1, and cis-9 cis-12 cis-15 C18:3 were positively influenced by omasal proportion of their respective FA but negatively related to calculated energy balance. Our findings demonstrate that for most milk FA examined, a larger variation is attributed to diet than b-cow differences with low to moderate repeatability. While some milk FA were positively or negatively related with Y_CH4VFA, there was a pronounced effect of calculated energy balance on these estimates. Additionally, even though OBCFA have been indicated as markers of rumen function, our results suggest that endogenous synthesis of these FA may occur, which therefore, may limit the utilization of milk FA as a proxy for CH4 predictions for cows fed the same diet.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Agricultural production / Animal and Dairy Science / Animal Feed / Animal lactation / Animal sciences / Animals / Biological Variation, Population / Biology and Life Sciences / Biosynthesis / Bivariate analysis / Cattle / Chain branching / Composition / Cow's milk / Dairy cattle / Dairy industry / Dairying / Datasets / Datasets as Topic / Diet / Emission analysis / Empirical analysis / Empirical models / Energy balance / Experiments / Fatty acids / Fatty Acids - analysis / Female / Fermentation / Food and nutrition / Greenhouse Effect / Health aspects / Husdjursvetenskap / Lactation - physiology / Mammary gland / Mammary glands / Medicine and Health Sciences / Methane / Methane - metabolism / Methane production / Milk / Milk - chemistry / Milk production / Physical Sciences / Physiological aspects / Physiology / Regression analysis / Regression models / Reproducibility / Research and Analysis Methods / Rumen / Rumen - metabolism / Standard deviation / Stoichiometry / Synthesis / Variation / Zoology