Journal Article

Tubulin Polyglutamylase Enzymes Are Members of the TTL Domain Protein Family

Gaertig, Jacek, Janke, Carsten, Suryavanshi, Swati, Wloga, Dorota, Regnard, Catherine, Strub, Jean-Marc, Eddé, Bernard, Rogowski, Krzysztof, Kajava, Andrey V, van Dijk, Juliette, Temurak, Nevzat, Boucher, Dominique, van Dorsselaer, Alain

2005

Overview

Polyglutamylation of tubulin has been implicated in several functions of microtubules, but the identification of the responsible enzyme(s) has been challenging. We found that the neuronal tubulin polyglutamylase is a protein complex containing a tubulin tyrosine ligase-like (TTLL) protein, TTLL1. TTLL1 is a member of a large family of proteins with a TTL homology domain, whose members could catalyze ligations of diverse amino acids to tubulins or other substrates. In the model protist Tetrahymena thermophila, two conserved types of polyglutamylases were characterized that differ in substrate preference and subcellular localization.

• Publisher: American Association for the Advancement of Science,The American Association for the Advancement of Science
• Subject: Amino Acid Motifs / Amino Acid Sequence / Amino acids / Analytical, structural and metabolic biochemistry / Animals / Antibodies / Binding Sites / Biochemistry / Biological and medical sciences / Brain - enzymology / Cadmium / Catalytic Domain / Cell growth / Cell motility / Cellular immunity / Cilia / Cilia - physiology / Enzymes / Enzymes and enzyme inhibitors / Fundamental and applied biological sciences. Psychology / Glutamate / Humans / Ligases / Mice / microtubules / Microtubules - metabolism / Models, Molecular / Molecular Sequence Data / Movement / neurons / Peptide Synthases - chemistry / Peptide Synthases - genetics / Peptide Synthases - isolation & purification / Peptide Synthases - metabolism / Phylogeny / Polyglutamic Acid - chemistry / Polyglutamic Acid - genetics / Polyglutamic Acid - isolation & purification / Polyglutamic Acid - metabolism / Protein Conformation / Protein Subunits - chemistry / Protein Subunits - isolation & purification / Protein Subunits - metabolism / Proteins / protists / Recombinant Fusion Proteins - metabolism / sequence homology / Substrate Specificity / Tetrahymena thermophila / Tetrahymena thermophila - enzymology / Tetrahymena thermophila - genetics / Tetrahymena thermophila - metabolism / tubulin / Tubulin - chemistry / Tubulin - genetics / Tubulin - isolation & purification / Tubulin - metabolism / tyrosine