Journal Article

Ablation of non-coding RNAs affects bovine leukemia virus B lymphocyte proliferation and abrogates oncogenesis

Safari, Roghaiyeh, Willems, Luc, Brostaux, Yves, Jacques, Jean-Rock

2020

Overview

Viruses have developed different strategies to escape from immune response. Among these, viral non-coding RNAs are invisible to the immune system and may affect the fate of the host cell. Bovine leukemia virus (BLV) encodes both short (miRNAs) and long (antisense AS1 and AS2) non-coding RNAs. To elucidate the mechanisms associated with BLV non-coding RNAs, we performed phenotypic and transcriptomic analyzes in a reverse genetics system. RNA sequencing of B-lymphocytes revealed that cell proliferation is the most significant mechanism associated with ablation of the viral non-coding RNAs. To assess the biological relevance of this observation, we determined the cell kinetic parameters in vivo using intravenous injection of BrdU and CFSE. Fitting the data to a mathematical model provided the rates of cell proliferation and death. Our data show that deletion of miRNAs correlates with reduced proliferation of the infected cell and lack of pathogenesis.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Ablation / Animals / Apoptosis / Binding sites / Biology and Life Sciences / Bovine leukosis / Cell death / Cell proliferation / Cellular biology / Epigenetics / Gammaretroviruses / Gene expression / Gene sequencing / Genetic aspects / Genetics / Health aspects / Human health sciences / Immune response / Immune system / In vivo methods and tests / Infections / Intravenous administration / Leukemia / Lymphocytes / Lymphocytes B / Lymphoma / Mathematical models / Medicine and Health Sciences / Methods / MicroRNAs / Non-coding RNA / Oncologie / Oncology / Pathogenesis / Principal components analysis / Research and analysis methods / Ribonucleic acid / RNA / RNA polymerase / RNA sequencing / Sciences de la santé humaine / Sheep / Transcriptomics / Tumorigenesis / Viruses