Journal Article

Cytotoxicity activities and chemical characteristics of exopolysaccharides and intracellular polysaccharides of Physarum polycephalum microplasmodia

Lai, Tran N. B., Do, Tuyen T. H., Stephenson, Steven L., Tran, Hanh T. M.

2021

Overview

Background Microbial polysaccharides have been reported to possess remarkable bioactivities. Physarum polycephalum is a species of slime mold for which the microplasmodia are capable of rapid growth and can produce a significant amount of cell wall-less biomass. There has been a limited understanding of the polysaccharides produced by microplasmodia of slime molds, including P. polycephalum . Thus, the primary objectives of this research were first to chemically characterize the exopolysaccharides (EPS) and intracellular polysaccharides (IPS) of P. polycephalum microplasmodia and then to evaluate their cytotoxicity against several cancer cell lines. Results The yields of the crude EPS (4.43 ± 0.44 g/l) and partially purified (deproteinated) EPS (2.95  ±  0.85 g/l) were comparable ( p  > 0.05) with the respective crude IPS (3.46 ± 0.36 g/l) and partially purified IPS (2.45 ± 0.36 g/l). The average molecular weight of the EPS and IPS were 14,762 kDa and 1788 kDa. The major monomer of the EPS was galactose (80.22%), while that of the IPS was glucose (84.46%). Both crude and purified IPS samples showed significantly higher cytotoxicity toward Hela cells, especially the purified sample and none of the IPSs inhibited normal cells. Only 38.42 ± 2.84% Hela cells remained viable when treated with the partially purified IPS (1 mg/ml). However, although only 34.76 ± 6.58% MCF-7 cells were viable when exposed to the crude IPS, but the partially purified IPS displayed non-toxicity to MCF-7 cells. This suggested that the cytotoxicity toward MCF-7 would come from some component associated with the crude IPS sample (e.g. proteins, peptides or ion metals) and the purification process would have either completely removed or reduced amount of that component. Cell cycle analysis by flow cytometry suggested that the mechanism of the toxicity of the crude IPS toward MCF-7 and the partially purified IPS toward Hela cells was due to apoptosis. Conclusions The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities. The crude and partially purified IPSs would be potential materials for further study relating to cancer treatment.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Antibiotics / Antineoplastic Agents - chemistry / Antineoplastic Agents - metabolism / Antineoplastic Agents - pharmacokinetics / Apoptosis / Applied Microbiology / Biochemical Engineering / Biocompatibility / biomass / Biomedical Engineering/Biotechnology / Biotechnology / Cancer / cancer therapy / Carbohydrates / Cell cycle / Cell walls / Chemical properties / Chemistry / Chemistry and Materials Science / Chemotherapy / Cytotoxicity / Exopolysaccharides / Flow cytometry / Fourier transforms / Galactose / Genetic Engineering / glucose / HeLa / HeLa Cells / Humans / Intracellular / MCF-7 / MCF-7 Cells / Metals / Microorganisms / Microplasmodia / Molecular Weight / Monosaccharides / Myxomycetes / neoplasm cells / Peptides / Physarum polycephalum / Physarum polycephalum - chemistry / Physarum polycephalum - metabolism / Physiological aspects / Plant Breeding/Biotechnology / Polysaccharides / Polysaccharides - chemistry / Polysaccharides - metabolism / Polysaccharides - pharmacology / Protein purification / Proteins / purification methods / Research Article / Saccharides / Slime / Slime molds / Spectrum analysis / sulfates / Sulfur content / Toxicity / Tumor cell lines