Journal Article

ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

Solana, Jordi, Kenny, Nathan J., Sleight, Victoria A., Neiro, Jakke, Iglesias, Marta, Schönauer, Anna, Álvarez-Campos, Patricia, Permanyer, Jon, Elek, Anamaria, Aboobaker, Aziz, Irimia, Manuel, Mason, Vincent, Sebé-Pedrós, Arnau, García-Castro, Helena

2021

Overview

Single-cell sequencing technologies are revolutionizing biology, but they are limited by the need to dissociate live samples. Here, we present ACME (ACetic-MEthanol), a dissociation approach for single-cell transcriptomics that simultaneously fixes cells. ACME-dissociated cells have high RNA integrity, can be cryopreserved multiple times, and are sortable and permeable. As a proof of principle, we provide single-cell transcriptomic data of different species, using both droplet-based and combinatorial barcoding single-cell methods. ACME uses affordable reagents, can be done in most laboratories and even in the field, and thus will accelerate our knowledge of cell types across the tree of life.

• Publisher: BioMed Central,Springer Nature B.V,BMC
• Subject: Animal Genetics and Genomics / Animals / Bioinformatics / Biomedical and Life Sciences / Cryopreservation / Cytoplasm / Deoxyribonucleic acid / Dissociation / DNA / Evolutionary Biology / Fixation / Flow cytometry / Gene expression / Gene Expression Profiling - methods / Gene Expression Profiling - standards / genome / Glycerol / High-Throughput Nucleotide Sequencing / Human Genetics / Life Sciences / Method / Methods / Microbial Genetics and Genomics / Morphology / Planarian / Planarians - cytology / Planarians - genetics / Plant Genetics and Genomics / Population / RNA / RNA-seq / Sequence Analysis, RNA / Single-Cell Analysis - methods / Single-Cell Analysis - standards / Single-cell transcriptomics / species / SPLiT-seq / Transcriptome / Transcriptomics / Workflow