Journal Article

Autophagic cell death associated to Sorafenib in renal cell carcinoma is mediated through Akt inhibition in an ERK1/2 independent fashion

Belandia, Borja, Pascual-Serra, Raquel, Giménez-Bachs, José M., Ortega-Muelas, Marta, Sanchez-Prieto, Ricardo, García-Cano, Jesús, Valero, María Ll, Cimas, Francisco J., Roche, Olga, Fernandez-Aroca, Diego M., Ruiz-Hidalgo, María J., Salinas, Antonio S., Serrano-Oviedo, Leticia

2018

Overview

To fully clarify the role of Mitogen Activated Protein Kinase in the therapeutic response to Sorafenib in Renal Cell Carcinoma as well as the cell death mechanism associated to this kinase inhibitor, we have evaluated the implication of several Mitogen Activated Protein Kinases in Renal Cell Carcinoma-derived cell lines. An experimental model of Renal Cell Carcinoma-derived cell lines (ACHN and 786-O cells) was evaluated in terms of viability by MTT assay, induction of apoptosis by caspase 3/7 activity, autophagy induction by LC3 lipidation, and p62 degradation and kinase activity using phospho-targeted antibodies. Knock down of ATG5 and ERK5 was performed using lentiviral vector coding specific shRNA. Our data discard Extracellular Regulated Kinase 1/2 and 5 as well as p38 Mitogen Activated Protein Kinase pathways as mediators of Sorafenib toxic effect but instead indicate that the inhibitory effect is exerted through the PI3K/Akt signalling pathway. Furthermore, we demonstrate that inhibition of Akt mediates cell death associated to Sorafenib without caspase activation, and this is consistent with the induction of autophagy, as indicated by the use of pharmacological and genetic approaches. The present report demonstrates that Sorafenib exerts its toxic effect through the induction of autophagy in an Akt-dependent fashion without the implication of Mitogen Activated Protein Kinase. Therefore, our data discard the use of inhibitors of the RAF-MEK-ERK1/2 signalling pathway in RCC and support the use of pro-autophagic compounds, opening new therapeutic opportunities for Renal Cell Carcinoma.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: 1-Phosphatidylinositol 3-kinase / AKT protein / Analysis / Antibodies / Apoptosis / Autophagy / Biology and Life Sciences / Biotechnology / Cancer therapies / Care and treatment / Caspase / Caspase-3 / Cell cycle / Cell death / Chemical compounds / Dosage and administration / Enzyme inhibitors / Extracellular signal-regulated kinase / Health aspects / Inhibition / Inhibitor drugs / Kidney cancer / Kinases / Liver cancer / Medicine and Health Sciences / Mitogens / Mortality / Phagocytosis / Pharmacology / Protein kinase / Protein kinases / Proteins / Raf protein / Renal cell carcinoma / Research and analysis methods / Signal transduction / Signaling / Sorafenib / Targeted cancer therapy / Tumor cell lines / Viability