Journal Article

Dopamine Receptor Activation Reorganizes Neuronal Ensembles during Hippocampal Sharp Waves In Vitro

Miyawaki, Takeyuki, Matsuki, Norio, Ikegaya, Yuji, Watanabe, Yusuke, Ishikawa, Tomoe, Norimoto, Hiroaki

2014

Overview

Hippocampal sharp wave (SW)/ripple complexes are thought to contribute to memory consolidation. Previous studies suggest that behavioral rewards facilitate SW occurrence in vivo. However, little is known about the precise mechanism underlying this enhancement. Here, we examined the effect of dopaminergic neuromodulation on spontaneously occurring SWs in acute hippocampal slices. Local field potentials were recorded from the CA1 region. A brief (1 min) treatment with dopamine led to a persistent increase in the event frequency and the magnitude of SWs. This effect lasted at least for our recording period of 45 min and did not occur in the presence of a dopamine D1/D5 receptor antagonist. Functional multineuron calcium imaging revealed that dopamine-induced SW augmentation was associated with an enriched repertoire of the firing patterns in SW events, whereas the overall tendency of individual neurons to participate in SWs and the mean number of cells participating in a single SW were maintained. Therefore, dopaminergic activation is likely to reorganize cell assemblies during SWs.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Action Potentials - drug effects / Activation / Animals / Augmentation / Biology and Life Sciences / Brain slice preparation / Calcium / Calcium imaging / Calcium signalling / Consolidation / Dopamine / Dopamine - administration & dosage / Dopamine - metabolism / Dopamine D1 receptors / Dopamine D5 receptors / Fourier transforms / Hippocampus / Hippocampus - drug effects / Hippocampus - metabolism / Hippocampus - physiology / In vivo methods and tests / Laboratories / Memory - drug effects / Memory - physiology / Mice / Neural networks / Neuromodulation / Neurons / Neurons - drug effects / Neurons - physiology / Pharmaceutical sciences / Pharmacology / Phenols (Class of compounds) / Receptor mechanisms / Receptors, Dopamine D5 - antagonists & inhibitors / Rodents / Schizophrenia