Asset Details
Do you wish to reserve the book?
Manipulating the Sensitivity of Signal-Induced Repression: Quantification and Consequences of Altered Brinker Gradients
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Manipulating the Sensitivity of Signal-Induced Repression: Quantification and Consequences of Altered Brinker Gradients
Do you wish to request the book?
Manipulating the Sensitivity of Signal-Induced Repression: Quantification and Consequences of Altered Brinker Gradients
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Manipulating the Sensitivity of Signal-Induced Repression: Quantification and Consequences of Altered Brinker Gradients
2013
Overview
Traditionally, the analysis of gene regulatory regions suffered from the caveat that it was restricted to artificial contexts (e.g. reporter constructs of limited size). With the advent of the BAC recombineering technique, genomic constructs can now be generated to test regulatory elements in their endogenous environment. The expression of the transcriptional repressor brinker (brk) is negatively regulated by Dpp signaling. Repression is mediated by small sequence motifs, the silencer elements (SEs), that are present in multiple copies in the regulatory region of brk. In this work, we manipulated the SEs in the brk locus. We precisely quantified the effects of the individual SEs on the Brk gradient in the wing disc by employing a 1D data extraction method, followed by the quantification of the data with reference to an internal control. We found that mutating the SEs results in an expansion of the brk expression domain. However, even after mutating all predicted SEs, repression could still be observed in regions of maximal Dpp levels. Thus, our data point to the presence of additional, low affinity binding sites in the brk locus.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Bacterial artificial chromosomes
/ Biology
/ Drosophila - growth & development
/ Drosophila Proteins - analysis
/ Drosophila Proteins - genetics
/ Drosophila Proteins - metabolism
/ Gene Expression Regulation, Developmental
/ Genes
/ Green Fluorescent Proteins - analysis
/ Green Fluorescent Proteins - genetics
/ Insects
/ Loci
/ Mutation
/ Proteins
/ Repressor Proteins - analysis
/ Repressor Proteins - genetics
/ Silencer Elements, Transcriptional
