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Large-Scale Biomonitoring of Remote and Threatened Ecosystems via High-Throughput Sequencing
by
Hajibabaei, Mehrdad
, Gibson, Joel F.
, Shokralla, Shadi
, Curry, Colin
, Monk, Wendy A.
, Baird, Donald J.
, King, Ian
in
Analysis
/ Animals
/ Anthropogenic factors
/ Biodiversity
/ Bioindicators
/ Biological monitoring
/ Biology
/ Biomonitoring
/ Cost analysis
/ Deoxyribonucleic acid
/ DNA
/ DNA Barcoding, Taxonomic
/ DNA sequencing
/ Ecological monitoring
/ Ecosystem
/ Ecosystem assessment
/ Ecosystem biology
/ Ecosystems
/ End users
/ Environmental monitoring
/ Environmental Monitoring - methods
/ Environmental monitoring programs
/ Fresh Water
/ Gene sequencing
/ High-Throughput Nucleotide Sequencing - methods
/ Identification
/ Identification methods
/ Invertebrates - classification
/ Invertebrates - genetics
/ Morphology
/ Multiculturalism & pluralism
/ National parks
/ Nucleotide sequence
/ Oil sands
/ Parks & recreation areas
/ Remote Sensing Technology
/ Rivers
/ Sequence Analysis, DNA - methods
/ Taxonomy
/ User requirements
2015
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Large-Scale Biomonitoring of Remote and Threatened Ecosystems via High-Throughput Sequencing
by
Hajibabaei, Mehrdad
, Gibson, Joel F.
, Shokralla, Shadi
, Curry, Colin
, Monk, Wendy A.
, Baird, Donald J.
, King, Ian
in
Analysis
/ Animals
/ Anthropogenic factors
/ Biodiversity
/ Bioindicators
/ Biological monitoring
/ Biology
/ Biomonitoring
/ Cost analysis
/ Deoxyribonucleic acid
/ DNA
/ DNA Barcoding, Taxonomic
/ DNA sequencing
/ Ecological monitoring
/ Ecosystem
/ Ecosystem assessment
/ Ecosystem biology
/ Ecosystems
/ End users
/ Environmental monitoring
/ Environmental Monitoring - methods
/ Environmental monitoring programs
/ Fresh Water
/ Gene sequencing
/ High-Throughput Nucleotide Sequencing - methods
/ Identification
/ Identification methods
/ Invertebrates - classification
/ Invertebrates - genetics
/ Morphology
/ Multiculturalism & pluralism
/ National parks
/ Nucleotide sequence
/ Oil sands
/ Parks & recreation areas
/ Remote Sensing Technology
/ Rivers
/ Sequence Analysis, DNA - methods
/ Taxonomy
/ User requirements
2015
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Do you wish to request the book?
Large-Scale Biomonitoring of Remote and Threatened Ecosystems via High-Throughput Sequencing
by
Hajibabaei, Mehrdad
, Gibson, Joel F.
, Shokralla, Shadi
, Curry, Colin
, Monk, Wendy A.
, Baird, Donald J.
, King, Ian
in
Analysis
/ Animals
/ Anthropogenic factors
/ Biodiversity
/ Bioindicators
/ Biological monitoring
/ Biology
/ Biomonitoring
/ Cost analysis
/ Deoxyribonucleic acid
/ DNA
/ DNA Barcoding, Taxonomic
/ DNA sequencing
/ Ecological monitoring
/ Ecosystem
/ Ecosystem assessment
/ Ecosystem biology
/ Ecosystems
/ End users
/ Environmental monitoring
/ Environmental Monitoring - methods
/ Environmental monitoring programs
/ Fresh Water
/ Gene sequencing
/ High-Throughput Nucleotide Sequencing - methods
/ Identification
/ Identification methods
/ Invertebrates - classification
/ Invertebrates - genetics
/ Morphology
/ Multiculturalism & pluralism
/ National parks
/ Nucleotide sequence
/ Oil sands
/ Parks & recreation areas
/ Remote Sensing Technology
/ Rivers
/ Sequence Analysis, DNA - methods
/ Taxonomy
/ User requirements
2015
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Large-Scale Biomonitoring of Remote and Threatened Ecosystems via High-Throughput Sequencing
Journal Article
Large-Scale Biomonitoring of Remote and Threatened Ecosystems via High-Throughput Sequencing
2015
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Overview
Biodiversity metrics are critical for assessment and monitoring of ecosystems threatened by anthropogenic stressors. Existing sorting and identification methods are too expensive and labour-intensive to be scaled up to meet management needs. Alternately, a high-throughput DNA sequencing approach could be used to determine biodiversity metrics from bulk environmental samples collected as part of a large-scale biomonitoring program. Here we show that both morphological and DNA sequence-based analyses are suitable for recovery of individual taxonomic richness, estimation of proportional abundance, and calculation of biodiversity metrics using a set of 24 benthic samples collected in the Peace-Athabasca Delta region of Canada. The high-throughput sequencing approach was able to recover all metrics with a higher degree of taxonomic resolution than morphological analysis. The reduced cost and increased capacity of DNA sequence-based approaches will finally allow environmental monitoring programs to operate at the geographical and temporal scale required by industrial and regulatory end-users.
Publisher
Public Library of Science,Public Library of Science (PLoS)
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