Journal Article

Effect of different levels of feed restriction and fish oil fatty acid supplementation on fat deposition by using different techniques, plasma levels and mRNA expression of several adipokines in broiler breeder hens

Dupont, Joëlle, Chartrin, Pascal, Pôle d'Expérimentation Avicole de Tours (UE PEAT) ; Institut National de la Recherche Agronomique (INRA), Lecompte, François, Venturi, Eric, Travel, Angélique, Ramé, Christelle, Physiologie de la reproduction et des comportements [Nouzilly] (PRC) ; Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Froment, Pascal, Biologie des Oiseaux et Aviculture (BOA) ; Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Institut Technique de l'AVIculture (ITAVI), Mercerand, Frédéric, Région Centre Val de Loire grant "Adipofertikines" N ̊ 32000407;MENRT, Mellouk, Namya, Marchand, Maxime, Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO) ; Institut National de la Recherche Agronomique (INRA), Touze, Jean-Luc, Ma, Lin-Lin, Staub, Christophe

2018

Overview

Reproductive hens are subjected to a restricted diet to limit the decline in fertility associated with change in body mass. However, endocrine and tissue responses to diet restriction need to be documented. We evaluated the effect of different levels of feed restriction, with or without fish oil supplementation, on metabolic parameters and adipokine levels in plasma and metabolic tissues of reproductive hens. We designed an in vivo protocol involving 4 groups of hens; RNS: restricted (Rt) unsupplemented, ANS: ad libitum (Ad, receiving an amount of feed 1.7 times greater than animals on the restricted diet) unsupplemented, RS: Rt supplemented, and AS: Ad supplemented. The fish oil supplement was used at 1% of the total diet composition. Hens fed with the Rt diet had a significantly (P < 0.0001) lower growth than Ad hens, while the fish oil supplementation had no effect on these parameters. Furthermore, the bioelectrical impedance analysis (BIA) and the fat ultrasonographic examinations produced similar results to the other methods that required animals to be killed (carcass analysis and weight of adipose tissue). In addition, the Rt diet significantly (P < 0.05) decreased plasma levels of triglycerides, phospholipids, glucose and ADIPOQ, and fish oil supplementation decreased plasma levels of RARRES2. We also showed a positive correlation between insulin values and ADIPOQ or NAMPT or RARRES2 values, and a negative correlation of fat percentage to RARRES2 values. Moreover, the effects of the Rt diet and fish oil supplementation on the mRNA expression depended on the factors tested and the hen age. Rt diet and fish oil supplementation are able to modulate metabolic parameters and the expression of adipokines and their receptors in metabolic tissue.

• Publisher: CCSD,Public Library of Science,Public Library of Science (PLoS)
• Subject: Adipose tissue / Analysis / Animal tissues / Animals / Bioelectricity / Biology and Life Sciences / Body mass / Broilers (Chickens) / Correlation / Diet / Diet therapy / Dietary restrictions / Dietary supplements / Fatty acids / Feed additives / Fertility / Fish / Fish oils / Food and Nutrition / Gene expression / Health aspects / In vivo methods and tests / Insulin / Insulin-like growth factors / Life Sciences / Medicine and Health Sciences / Oils & fats / Other / Phospholipids / Physiological aspects / Plasma levels / Poultry / Receptors / Rodents / Triglycerides
• ISBN: 0004234125000